干旱区生态环境知识资源中心(Arid): Development of a unified method for the determination of legacy and metabolites of current pesticides in serum for exposure assessment

Arid

DOI	10.1007/s00216-024-05488-2
	Development of a unified method for the determination of legacy and metabolites of current pesticides in serum for exposure assessment
	Birolli, Willian G.; Lancas, Fernando M.; Silveira, Henrique C. S.; Santos-neto, Alvaro J.
通讯作者	Birolli, WG
来源期刊	ANALYTICAL AND BIOANALYTICAL CHEMISTRY
ISSN	1618-2642
EISSN	1618-2650
出版年	2024
英文摘要	The use of pesticides is often regarded as a fundamental aspect of conventional agriculture. However, these compounds have gained recognition as some of the oldest and most widely employed xenobiotic contaminants, necessitating effective strategies for human biomonitoring. In this context, a method was developed for the determination of 16 legacy organochlorine pesticides, 6 metabolites of current pesticides (2,4-D, malathion, parathion, fipronil, pyraclostrobin, cypermethrin, permethrin, cyfluthrin), and 1 triazine herbicide (atrazine) in serum. Samples were prepared with water, formic acid, acetonitrile, and ultrasound irradiation, followed by solid-phase extraction with Oasis Prime HLB. Subsequently, metabolites from current pesticides underwent derivatization using MTBSTFA with 1% TBDMSCl for analysis via gas chromatography-tandem mass spectrometry (GC-MS/MS), employing an SLB-5MS fused silica capillary column. Analytical curves were generated with limits of quantification from 0.3 to 4.0 ng.mL-1. Accuracy ranged from 69 to 124%, and the coefficient of variation from 2 to 28%. Moreover, determining 1-(4-chlorophenyl)-1H-pyrazol-3-ol was suggested as a biomarker for pyraclostrobin biomonitoring. This analytical approach facilitated the determination of both legacy and metabolites of current pesticides in the same serum sample, presenting an interesting and cost-effective option for large cohorts, and multi-omics studies that evaluate time-dependent biomarkers in blood samples, thereby enabling biomonitoring within the same matrix. Furthermore, a proof-of-concept involving 10 volunteers demonstrated exposure to 9 pesticides at mean concentrations measured in ng mL-1, consistent with findings from various biomonitoring initiatives.
英文关键词	Persistent organic pollutants Biomonitoring Organophosphate Pyrethroid Gas chromatography Mass spectrometry
类型	Article ; Early Access
语种	英语
收录类别	SCI-E
WOS记录号	WOS:001298747600002
WOS关键词	SOLID-PHASE EXTRACTION ; GAS-CHROMATOGRAPHY ; ORGANOCHLORINE PESTICIDES ; URINARY METABOLITES
WOS类目	Biochemical Research Methods ; Chemistry, Analytical
WOS研究方向	Biochemistry & Molecular Biology ; Chemistry
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/402819
推荐引用方式 GB/T 7714	Birolli, Willian G.,Lancas, Fernando M.,Silveira, Henrique C. S.,et al. Development of a unified method for the determination of legacy and metabolites of current pesticides in serum for exposure assessment[J],2024.
APA	Birolli, Willian G.,Lancas, Fernando M.,Silveira, Henrique C. S.,&Santos-neto, Alvaro J..(2024).Development of a unified method for the determination of legacy and metabolites of current pesticides in serum for exposure assessment.ANALYTICAL AND BIOANALYTICAL CHEMISTRY.
MLA	Birolli, Willian G.,et al."Development of a unified method for the determination of legacy and metabolites of current pesticides in serum for exposure assessment".ANALYTICAL AND BIOANALYTICAL CHEMISTRY (2024).