干旱区生态环境知识资源中心(Arid): Novel, alternative analytical methodology for determination of antimicrobial chemicals in aquatic environments and public use assessment: Extraction sorbent, microbiological sensitivity, stability, and applicability

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DOI	10.1016/j.aca.2023.342029
	Novel, alternative analytical methodology for determination of antimicrobial chemicals in aquatic environments and public use assessment: Extraction sorbent, microbiological sensitivity, stability, and applicability
	Ugolini, Valentina; Lai, Foon Yin
通讯作者	Ugolini, V ; Lai, FY
来源期刊	ANALYTICA CHIMICA ACTA
ISSN	0003-2670
EISSN	1873-4324
出版年	2024
卷号	1286
英文摘要	Background: Assessing antimicrobial chemicals from wastewater source to recipient water systems is crucial in planning effective, policy-related interventions for antimicrobial resistance (AMR) risk mitigation. However, the capability of related analytical methods for AMR assessment has not been explored previously. There is also a lack of knowledge on the effectiveness of alternative extraction sorbents with ion-exchange functions, and little information on chemical stability from sampling to analysis as well as preservative options. Hence, our study aims to address the clear need for advanced, broad-range and microbiologically-sensitive methodologies, paired with thorough stability assessments.Results: Oasis (R) WCX ion-exchange was for the first time employed in solid-phase extraction (SPE) for antibacterials, antifungals, antivirals and human metabolites in various water matrices. Analysis was performed using liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS) on a biphenyl analytical column. The optimized and validated method provided satisfactory accuracy, precision, and recovery for 53 compounds via LC-MS/MS direct injection and for up to 35 compounds via SPE-LC-MS/MS. Method quantification limits (MQLs) were determined in groundwater (0.33-54 ng L-1), surface water (0.53-75 ng L-1), effluent wastewater (2.5-470 ng L-1), and influent wastewater (11-650 ng L-1). As a novel approach, MQLs were compared with minimum inhibitory concentrations, to confirm our method's microbiological sensitivity for studying AMR. Stability assessment revealed that most compounds remained stable in standard solution at-80 degrees C for six months, in various waters at-20 degrees C for eight weeks, and during 24-h sampling at 4 degrees C. Sodium azide was a better preservative than sodium metabisulfite.Significance: Our study is an added value to the analytical methodology for water measurements of antimicrobial chemicals, in which it provides a novel, alternative method that is robust and overall more sensitive than others using generic Oasis (R) HLB sorbents and C18 analytical columns in SPE-LC-MS/MS. Also, the comprehensive data on antimicrobial stability helps reduce methodological uncertainty for future studies. Our method shows sufficient microbiologically-sensitivity and thus is suitable for future (inter)national regulatory water monitoring of AMR.
英文关键词	Emerging contaminants Antimicrobial resistance Minimum inhibitory concentrations Preservative agents
类型	Article
语种	英语
开放获取类型	Green Published, hybrid
收录类别	SCI-E
WOS记录号	WOS:001127847300001
WOS关键词	SOLID-PHASE EXTRACTION ; PERFORMANCE LIQUID-CHROMATOGRAPHY ; TANDEM MASS-SPECTROMETRY ; URBAN WASTE-WATER ; DISINFECTION BY-PRODUCTS ; TETRACYCLINE ANTIBIOTICS ; POPULATION EXPOSURE ; MULTIPLE CLASSES ; RAPID ANALYSIS ; SURFACE-WATER
WOS类目	Chemistry, Analytical
WOS研究方向	Chemistry
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/402817
推荐引用方式 GB/T 7714	Ugolini, Valentina,Lai, Foon Yin. Novel, alternative analytical methodology for determination of antimicrobial chemicals in aquatic environments and public use assessment: Extraction sorbent, microbiological sensitivity, stability, and applicability[J],2024,1286.
APA	Ugolini, Valentina,&Lai, Foon Yin.(2024).Novel, alternative analytical methodology for determination of antimicrobial chemicals in aquatic environments and public use assessment: Extraction sorbent, microbiological sensitivity, stability, and applicability.ANALYTICA CHIMICA ACTA,1286.
MLA	Ugolini, Valentina,et al."Novel, alternative analytical methodology for determination of antimicrobial chemicals in aquatic environments and public use assessment: Extraction sorbent, microbiological sensitivity, stability, and applicability".ANALYTICA CHIMICA ACTA 1286(2024).