干旱区生态环境知识资源中心(Arid): Halophilic Plant Growth-Promoting Rhizobacteria as Producers of Antifungal Metabolites under Salt Stress

Arid

DOI	10.3390/agronomy14040845
	Halophilic Plant Growth-Promoting Rhizobacteria as Producers of Antifungal Metabolites under Salt Stress
	Ouali, Karima Ould; Houali, Karim; Cruz, Cristina; Melo, Juliana; Benakli, Yasmina; Ousmer, Lila; Madani, Zahia; Nabti, El-Hafid
通讯作者	Nabti, E
来源期刊	AGRONOMY-BASEL
EISSN	2073-4395
出版年	2024
卷号	14 期号:4
英文摘要	Salinity is one of the main factors causing soil deterioration, making it unsuitable for agriculture. It is well documented that the application of halotolerant and halophilic plant growth-promoting bacteria (PGPR: plant growth-promoting rhizobacteria) with biological control activities as an inoculant of cultivated plants offers a biological alternative to the use of agrochemicals, particularly when subjected to salt stress. From this perspective, 70 bacterial strains were isolated from saline soils (sebkha) in arid and semi-arid areas of Eastern Algeria. Three isolates were selected based on their ability to produce bioactive molecules allowing them to promote plant growth, such as hydrolytic enzymes, indole acetic acid (auxin-phytohormone), HCN, NH3, etc. Two of these isolates belonged to the genus Serratia and the third was a halophilic Halomonas bacteria. These bacteria were identified based on their 16S rDNA sequences. Antagonism tests against phytopathogenic fungi were carried out. The identification of the antifungal molecules produced by these bacteria was determined using high-performance liquid chromatography. These bacteria can inhibit mycelial development against phytopathogenic fungi with rates reaching 80.67% against Botrytis cinerea, 76.22% against Aspergillus niger, and 66.67% against Fusarium culmorum for Serratia sp. The strain Halomonas sp. inhibited mycelial growth through the production of volatile substances of Aspergillus niger at 71.29%, Aspergillus flavus at 75.49%, and Penicillium glabrum at a rate of 72.22%. The identification of the antifungal molecules produced by these three bacteria using HPLC revealed that they were polyphenols, which makes these strains the first rhizobacteria capable of producing phenolic compounds. Finally, pot tests to determine the effectiveness of these strains in promoting wheat growth under salinity stress (125 mM, 150 mM, and 200 mM) was carried out. The results revealed that a consortium of two isolates (Serratia sp. and Halomonas sp.) performed best at 125 mM. However, at higher concentrations, it was the halophilic bacteria Halomonas sp. that gave the best result. In all cases, there was a significant improvement in the growth of wheat seedlings inoculated with the bacteria compared to non-inoculated controls.
英文关键词	halophilic bacteria salt stress biological control polyphenols plant growth-promoting bacteria (PGPB)
类型	Article
语种	英语
开放获取类型	gold
收录类别	SCI-E
WOS记录号	WOS:001221419300001
WOS关键词	WELL WATER ; BACTERIA ; SALINITY ; SOIL ; L. ; ALLEVIATION ; PRODIGIOSIN ; RESPONSES ; STRAINS ; TRAITS
WOS类目	Agronomy ; Plant Sciences
WOS研究方向	Agriculture ; Plant Sciences
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/402764
推荐引用方式 GB/T 7714	Ouali, Karima Ould,Houali, Karim,Cruz, Cristina,et al. Halophilic Plant Growth-Promoting Rhizobacteria as Producers of Antifungal Metabolites under Salt Stress[J],2024,14(4).
APA	Ouali, Karima Ould.,Houali, Karim.,Cruz, Cristina.,Melo, Juliana.,Benakli, Yasmina.,...&Nabti, El-Hafid.(2024).Halophilic Plant Growth-Promoting Rhizobacteria as Producers of Antifungal Metabolites under Salt Stress.AGRONOMY-BASEL,14(4).
MLA	Ouali, Karima Ould,et al."Halophilic Plant Growth-Promoting Rhizobacteria as Producers of Antifungal Metabolites under Salt Stress".AGRONOMY-BASEL 14.4(2024).