干旱区生态环境知识资源中心(Arid): Overexpressing ATP Sulfurylase Improves Fe-Deficiency Tolerance in Apple Calli and Tobacco

Arid

DOI	10.3390/agronomy14030404
	Overexpressing ATP Sulfurylase Improves Fe-Deficiency Tolerance in Apple Calli and Tobacco
	Cheng, Jiao; Zhang, Zhongxing; Gao, Yanlong; Dong, Yongjuan; Xian, Xulin; Li, Cailong; Ding, Liang; Wang, Yanxiu
通讯作者	Wang, YX
来源期刊	AGRONOMY-BASEL
EISSN	2073-4395
出版年	2024
卷号	14 期号:3
英文摘要	Iron (Fe) deficiency is one of the most common micronutrient deficiencies limiting crop production globally, especially in arid regions due to decreased availability of Fe in alkaline soils. The ATP sulfurylase (ATPS) gene has been reported to participate in regulating various abiotic stresses. Transcriptome data and qRT-PCR analysis revealed that the ATP sulfurylase gene MhATPS1 was notably induced by Fe-deficiency stress. Consequently, MhATPS1 (103410737) was isolated from Malus halliana, and transgenic tobacco and transgenic apple calli were successfully obtained by genetic transformation. Compared with the wild type (WT), transgenic MhATPS1 lines (transgenic tobacco and transgenic apple calli) displayed stronger resistance to Fe-deficiency treatment. To be specific, transgenic plants exhibited better growth, accumulated more Fe2+ content, had higher ferric chelate reductase (FCR) activity, and a greater active oxygen scavenging capacity. Furthermore, transgenic MhATPS1 lines up-regulated the expression of Fe uptake genes under Fe-deficit stress. Additionally, MhATPS1 transgenic lines secreted more H+ content compared to the WT. In summary, these findings indicate that the MhATPS1 gene may play a positive role in Fe-deficiency stress in both tobacco and apple calli.
英文关键词	ATP sulfurylase molecular cloning functional identification Fe-deficiency stress
类型	Article
语种	英语
开放获取类型	gold
收录类别	SCI-E
WOS记录号	WOS:001191614800001
WOS关键词	BHLH TRANSCRIPTION FACTOR ; IRON-DEFICIENCY ; PROTON EXTRUSION ; CULTIVARS ; RESPONSES ; STRESS ; ACCUMULATION ; ASSIMILATION ; TOXICITY ; CADMIUM
WOS类目	Agronomy ; Plant Sciences
WOS研究方向	Agriculture ; Plant Sciences
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/402754
推荐引用方式 GB/T 7714	Cheng, Jiao,Zhang, Zhongxing,Gao, Yanlong,et al. Overexpressing ATP Sulfurylase Improves Fe-Deficiency Tolerance in Apple Calli and Tobacco[J],2024,14(3).
APA	Cheng, Jiao.,Zhang, Zhongxing.,Gao, Yanlong.,Dong, Yongjuan.,Xian, Xulin.,...&Wang, Yanxiu.(2024).Overexpressing ATP Sulfurylase Improves Fe-Deficiency Tolerance in Apple Calli and Tobacco.AGRONOMY-BASEL,14(3).
MLA	Cheng, Jiao,et al."Overexpressing ATP Sulfurylase Improves Fe-Deficiency Tolerance in Apple Calli and Tobacco".AGRONOMY-BASEL 14.3(2024).