干旱区生态环境知识资源中心(Arid): Antimicrobial resistance patterns and characterisation of emerging beta-lactamase-producing Escherichia coli in camels sampled from Northern Kenya

Arid

DOI	10.1002/vms3.1090
	Antimicrobial resistance patterns and characterisation of emerging beta-lactamase-producing Escherichia coli in camels sampled from Northern Kenya
	Akunda, Irene Karegi; Kariuki, Daniel W.; Matulis, Graham; Mwaura, Patrick; Maina, Brian; Mohammed, Halima; Paul, Ayieko; Onyambu, Frank G.; Kwallah, Allan Ole; Martins, Dino J.; von Fricken, Michael E.; Kamau, Joseph M.
通讯作者	Akunda, IK
来源期刊	VETERINARY MEDICINE AND SCIENCE
EISSN	2053-1095
出版年	2023
卷号	9 期号:3 页码:1407-1416
英文摘要	BackgroundAnimal husbandry practices in different livestock production systems and increased livestock-wildlife interactions are thought to be primary drivers of antimicrobial resistance (AMR) in Arid and Semi-Arid Lands (ASALs). Despite a tenfold increase in the camel population within the last decade, paired with widespread use of camel products, there is a lack of comprehensive information concerning beta-lactamase-producing Escherichia coli (E. coli) within these production systems. ObjectivesOur study sought to establish an AMR profile and to identify and characterise emerging beta-lactamase-producing E. coli isolated from faecal samples obtained from camel herds in Northern Kenya. MethodsThe antimicrobial susceptibility profiles of E. coli isolates were established using the disk diffusion method, with beta-lactamase (bla) gene PCR product sequencing performed for phylogenetic grouping and genetic diversity assessments. ResultsHere we show, among the recovered E. coli isolates (n = 123), the highest level of resistance was observed for cefaclor at 28.5% of isolates, followed by cefotaxime at 16.3% and ampicillin at 9.7%. Moreover, extended-spectrum beta-lactamase (ESBL)-producing E. coli harbouring the bla(CTX-M-15) or bla(CTX-M-27) genes were detected in 3.3% of total samples, and are associated with phylogenetic groups B1, B2 and D. Multiple variants of non-ESBL bla(TEM) genes were detected, the majority of which were the bla(TEM-1) and bla(TEM-116) genes. ConclusionsFindings from this study shed light on the increased occurrence of ESBL- and non-ESBL-encoding gene variants in E. coli isolates with demonstrated multidrug resistant phenotypes. This study highlights the need for an expanded One Health approach to understanding AMR transmission dynamics, drivers of AMR development, and appropriate practices for antimicrobial stewardship in camel production systems within ASALs.
英文关键词	antimicrobial resistance beta-lactamase-producing Escherichia coli camels livestock production systems
类型	Article
语种	英语
开放获取类型	Green Published, gold
收录类别	SCI-E
WOS记录号	WOS:000937313000001
WOS关键词	ANIMALS ; FOOD ; LIVESTOCK ; BACTERIA ; PASTORALISTS ; MILK
WOS类目	Veterinary Sciences
WOS研究方向	Veterinary Sciences
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/398964
推荐引用方式 GB/T 7714	Akunda, Irene Karegi,Kariuki, Daniel W.,Matulis, Graham,et al. Antimicrobial resistance patterns and characterisation of emerging beta-lactamase-producing Escherichia coli in camels sampled from Northern Kenya[J],2023,9(3):1407-1416.
APA	Akunda, Irene Karegi.,Kariuki, Daniel W..,Matulis, Graham.,Mwaura, Patrick.,Maina, Brian.,...&Kamau, Joseph M..(2023).Antimicrobial resistance patterns and characterisation of emerging beta-lactamase-producing Escherichia coli in camels sampled from Northern Kenya.VETERINARY MEDICINE AND SCIENCE,9(3),1407-1416.
MLA	Akunda, Irene Karegi,et al."Antimicrobial resistance patterns and characterisation of emerging beta-lactamase-producing Escherichia coli in camels sampled from Northern Kenya".VETERINARY MEDICINE AND SCIENCE 9.3(2023):1407-1416.