干旱区生态环境知识资源中心(Arid): Development of High-Throughput Sample Preparation Procedures for the Quantitative Determination of Aflatoxins in Biological Matrices of Chickens and Cattle Using UHPLC-MS/MS

Arid

DOI	10.3390/toxins15010037
	Development of High-Throughput Sample Preparation Procedures for the Quantitative Determination of Aflatoxins in Biological Matrices of Chickens and Cattle Using UHPLC-MS/MS
	De Baere, Siegrid; Ochieng, Phillis E. E.; Kemboi, David C. C.; Scippo, Marie-Louise; Okoth, Sheila; Lindahl, Johanna F. F.; Gathumbi, James K. K.; Antonissen, Gunther; Croubels, Siska
通讯作者	De Baere, S
来源期刊	TOXINS
EISSN	2072-6651
出版年	2023
卷号	15 期号:1
英文摘要	Aflatoxins (AFs) frequently contaminate food and animal feeds, especially in (sub) tropical countries. If animals consume contaminated feeds, AFs (mainly aflatoxin B1 (AFB1), B2 (AFB2), G1 (AFG1), G2 (AFG2) and their major metabolites aflatoxin M1 (AFM1) and M2 (AFM2)) can be transferred to edible tissues and products, such as eggs, liver and muscle tissue and milk, which ultimately can reach the human food chain. Currently, the European Union has established a maximum level for AFM1 in milk (0.05 mu g kg(-1)). Dietary adsorbents, such as bentonite clay, have been used to reduce AFs exposure in animal husbandry and carry over to edible tissues and products. To investigate the efficacy of adding bentonite clay to animal diets in reducing the concentration of AFB1, AFB2, AFG1, AFG2, and the metabolites AFM1 and AFM2 in animal-derived foods (chicken muscle and liver, eggs, and cattle milk), chicken and cattle plasma and cattle ruminal fluid, a sensitive and selective ultra-high performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) method has been developed. High-throughput sample preparation procedures were optimized, allowing the analysis of 96 samples per analytical batch and consisted of a liquid extraction using 1% formic acid in acetonitrile, followed by a further clean-up using QuEChERS (muscle tissue), QuEChERS in combination with Oasis((R)) Ostro (liver tissue), Oasis((R)) Ostro (egg, plasma), and Oasis((R)) PRiME HLB (milk, ruminal fluid). The different procedures were validated in accordance with European guidelines. As a proof-of-concept, the final methods were used to successfully determine AFs concentrations in chicken and cattle samples collected during feeding trials for efficacy and safety evaluation of mycotoxin detoxifiers to protect against AFs as well as their carry-over to animal products.
英文关键词	aflatoxins chicken cattle UHPLC-MS/MS biological matrices residues food safety toxicokinetics
类型	Article
语种	英语
开放获取类型	Green Published, gold
收录类别	SCI-E
WOS记录号	WOS:000927773600001
WOS关键词	TANDEM MASS-SPECTROMETRY ; HUMAN BREAST-MILK ; MULTI-MYCOTOXIN ; ISOTOPE-DILUTION ; LIQUID ; OCHRATOXIN ; M1 ; BIOMARKERS ; PLASMA ; LIVER
WOS类目	Food Science & Technology ; Toxicology
WOS研究方向	Food Science & Technology ; Toxicology
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/398918
推荐引用方式 GB/T 7714	De Baere, Siegrid,Ochieng, Phillis E. E.,Kemboi, David C. C.,et al. Development of High-Throughput Sample Preparation Procedures for the Quantitative Determination of Aflatoxins in Biological Matrices of Chickens and Cattle Using UHPLC-MS/MS[J],2023,15(1).
APA	De Baere, Siegrid.,Ochieng, Phillis E. E..,Kemboi, David C. C..,Scippo, Marie-Louise.,Okoth, Sheila.,...&Croubels, Siska.(2023).Development of High-Throughput Sample Preparation Procedures for the Quantitative Determination of Aflatoxins in Biological Matrices of Chickens and Cattle Using UHPLC-MS/MS.TOXINS,15(1).
MLA	De Baere, Siegrid,et al."Development of High-Throughput Sample Preparation Procedures for the Quantitative Determination of Aflatoxins in Biological Matrices of Chickens and Cattle Using UHPLC-MS/MS".TOXINS 15.1(2023).