干旱区生态环境知识资源中心(Arid): Fine mapping of a Fusarium crown rot resistant locus on chromosome arm 6HL in barley by exploiting near isogenic lines, transcriptome profiling, and a large near isogenic line-derived population

Arid

DOI	10.1007/s00122-023-04387-x
	Fine mapping of a Fusarium crown rot resistant locus on chromosome arm 6HL in barley by exploiting near isogenic lines, transcriptome profiling, and a large near isogenic line-derived population
	Gao, Shang; Jiang, Yunfeng; Zhou, Hong; Liu, Yaxi; Li, Huihui; Liu, Chunji; Zheng, Zhi
通讯作者	Liu, CJ ; Zheng, Z
来源期刊	THEORETICAL AND APPLIED GENETICS
ISSN	0040-5752
EISSN	1432-2242
出版年	2023
卷号	136 期号:6
英文摘要	Key messageThis study reported validation and fine mapping of a Fusarium crown rot resistant locus on chromosome arm 6HL in barley using near isogenic lines, transcriptome sequences, and a large near isogenic line-derived population.Fusarium crown rot (FCR), caused by Fusarium pseudograminearum, is a chronic and serious disease affecting cereal production in semi-arid regions globally. The increasing prevalence of this disease in recent years is attributed to the widespread adoption of minimum tillage and stubble retention practices. In the study reported here, we generated eight pairs of near isogenic lines (NILs) targeting a putative QTL (Qcrs.caf-6H) conferring FCR resistance in barley. Assessing the NILs confirmed the large effect of this locus. Aimed to develop markers that can be reliably used in incorporating this resistant allele into breeding programs and identify candidate genes, transcriptomic analyses were conducted against three of the NIL pairs and a large NIL-derived population consisting of 1085 F7 recombinant inbred lines generated. By analyzing the transcriptomic data and the fine mapping population, Qcrs.caf-6H was delineated into an interval of 0.9 cM covering a physical distance of similar to 547 kb. Six markers co-segregating with this locus were developed. Based on differential gene expression and SNP variations between the two isolines among the three NIL pairs, candidate genes underlying the resistance at this locus were detected. These results would improve the efficiency of incorporating the targeted locus into barley breeding programs and facilitate the cloning of causal gene(s) responsible for the resistance.
类型	Article
语种	英语
开放获取类型	hybrid, Green Submitted, Green Published
收录类别	SCI-E
WOS记录号	WOS:000995498100002
WOS关键词	E3 UBIQUITIN LIGASE ; HEAD BLIGHT ; MAJOR QTL ; BREAD WHEAT ; CELL-DEATH ; RNA-SEQ ; PSEUDOGRAMINEARUM ; PATHOGENS ; DEFENSE ; IDENTIFICATION
WOS类目	Agronomy ; Plant Sciences ; Genetics & Heredity ; Horticulture
WOS研究方向	Agriculture ; Plant Sciences ; Genetics & Heredity
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/398910
推荐引用方式 GB/T 7714	Gao, Shang,Jiang, Yunfeng,Zhou, Hong,et al. Fine mapping of a Fusarium crown rot resistant locus on chromosome arm 6HL in barley by exploiting near isogenic lines, transcriptome profiling, and a large near isogenic line-derived population[J],2023,136(6).
APA	Gao, Shang.,Jiang, Yunfeng.,Zhou, Hong.,Liu, Yaxi.,Li, Huihui.,...&Zheng, Zhi.(2023).Fine mapping of a Fusarium crown rot resistant locus on chromosome arm 6HL in barley by exploiting near isogenic lines, transcriptome profiling, and a large near isogenic line-derived population.THEORETICAL AND APPLIED GENETICS,136(6).
MLA	Gao, Shang,et al."Fine mapping of a Fusarium crown rot resistant locus on chromosome arm 6HL in barley by exploiting near isogenic lines, transcriptome profiling, and a large near isogenic line-derived population".THEORETICAL AND APPLIED GENETICS 136.6(2023).