干旱区生态环境知识资源中心(Arid): Genetic diversity, symbiotic efficiency, stress tolerance, and plant growth promotion traits of rhizobia nodulating Vachellia tortilis subsp. raddiana growing in dryland soils in southern Morocco

Arid

DOI	10.1016/j.syapm.2023.126434
	Genetic diversity, symbiotic efficiency, stress tolerance, and plant growth promotion traits of rhizobia nodulating Vachellia tortilis subsp. raddiana growing in dryland soils in southern Morocco
	Hnini, Mohamed; El Attar, Imane; Taha, Kaoutar; Aurag, Jamal
通讯作者	Aurag, J
来源期刊	SYSTEMATIC AND APPLIED MICROBIOLOGY
ISSN	0723-2020
EISSN	1618-0984
出版年	2023
卷号	46 期号:4
英文摘要	In the present study, we analyzed the genetic diversity, phylogenetic relationships, stress tolerance, phytoben-eficial traits, and symbiotic characteristics of rhizobial strains isolated from root nodules of Vachellia tortilis subsp. raddiana grown in soils collected in the extreme Southwest of the Anti-Atlas Mountains in Morocco. Subsequent to Rep-PCR fingerprinting, 16S rDNA gene sequencing of 15 representative strains showed that all of them belong to the genus Ensifer. Phylogenetic analysis and concatenation of the housekeeping genes gyrB, rpoB, recA, and dnaK revealed that the entire collection (except strain LMR678) shared 99.08 % to 99.92% similarity with Ensifer sp. USDA 257 and 96.92% to 98.79% with Sinorhizobium BJ1. Phylogenetic anal-ysis of nodC and nodA sequences showed that all strains but one (LMR678) formed a phylogenetic group with the type strain E. aridi LMR001T (similarity over 98%). Moreover, it was relevant that most strains belong to the symbiovar vachelliae. In vitro tests revealed that five strains produced IAA, four solubilized inorganic phos-phate, and one produced siderophores. All strains showed tolerance to NaCl concentrations ranging from 2 to 12% and grew at up to 10% of PEG6000. A greenhouse plant inoculation test conducted during five months demonstrated that most rhizobial strains were infective and efficient. Strains LMR688, LMR692, and LMR687 exhibited high relative symbiotic efficiency values (respectively 231.6 %, 171.96 %, and 140.84 %). These strains could be considered as the most suitable candidates for inoculation of V. t. subsp. raddiana, to be used as a pioneer plant for restoring arid soils threatened with desertification.
英文关键词	Vachellia tortilis subsp raddiana Ensifer MLSA phylogeny Symbiotic effectiveness Plant growth promoting traits Stress tolerance
类型	Article
语种	英语
收录类别	SCI-E
WOS记录号	WOS:001012996400001
WOS关键词	MULTILOCUS SEQUENCE-ANALYSIS ; ARBUSCULAR MYCORRHIZAL FUNGI ; ENSIFER STRAINS ; ROOT-NODULES ; ACACIA ; PHYLOGENY ; REGIONS ; TREE ; 16S ; L.
WOS类目	Biotechnology & Applied Microbiology ; Microbiology
WOS研究方向	Biotechnology & Applied Microbiology ; Microbiology
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/398882
推荐引用方式 GB/T 7714	Hnini, Mohamed,El Attar, Imane,Taha, Kaoutar,et al. Genetic diversity, symbiotic efficiency, stress tolerance, and plant growth promotion traits of rhizobia nodulating Vachellia tortilis subsp. raddiana growing in dryland soils in southern Morocco[J],2023,46(4).
APA	Hnini, Mohamed,El Attar, Imane,Taha, Kaoutar,&Aurag, Jamal.(2023).Genetic diversity, symbiotic efficiency, stress tolerance, and plant growth promotion traits of rhizobia nodulating Vachellia tortilis subsp. raddiana growing in dryland soils in southern Morocco.SYSTEMATIC AND APPLIED MICROBIOLOGY,46(4).
MLA	Hnini, Mohamed,et al."Genetic diversity, symbiotic efficiency, stress tolerance, and plant growth promotion traits of rhizobia nodulating Vachellia tortilis subsp. raddiana growing in dryland soils in southern Morocco".SYSTEMATIC AND APPLIED MICROBIOLOGY 46.4(2023).