干旱区生态环境知识资源中心(Arid): Burkholderia semiarida sp. nov. and Burkholderia sola sp. nov., two novel B. cepacia complex species causing onion sour skin

Arid

DOI	10.1016/j.syapm.2023.126415
	Burkholderia semiarida sp. nov. and Burkholderia sola sp. nov., two novel B. cepacia complex species causing onion sour skin
	Velez, Leandro S.; Aburjaile, Flavia F.; Farias, Antonio R. G.; Baia, Ana D. B.; Oliveira, Willams J.; Silva, Adriano M. F.; Benko-Iseppon, Ana M.; Azevedo, Vasco; Brenig, Bertram; Ham, Jong H.; Souza, Elineide B.; Gama, Marco A. S.
通讯作者	Gama, MAS
来源期刊	SYSTEMATIC AND APPLIED MICROBIOLOGY
ISSN	0723-2020
EISSN	1618-0984
出版年	2023
卷号	46 期号:3
英文摘要	Two putative novel Burkholderia cenocepacia lineages found in the semi-arid region of north-east Brazil causing onion sour skin were studied using genomic approaches to determine their taxonomic position. Four strains belonging to one novel lineage (CCRMBC16, CCRMBC33, CCRMBC74, and CCRMBC171) and one strain (CCRMBC51) belonging to another novel lineage had their whole genome sequenced to carry out taxogenomic analyses. The phylogenomic tree built using the type (strain) genome server (TYGS) clustered the strains CCRMBC16, CCRMBC33, CCRMBC74, and CCRMBC171 into the same clade, while grouped the strain CCRMBC51 separately. Average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) analysis showed values above 99.21 % and 93.2 %, respectively, among the strains CCRMBC16, CCRMBC33, CCRMBC74, and CCRMBC171, while ANI and dDDH values between these strains and the strain CCRMBC51 were below 94.49 % and 56.6 %, respectively. All these strains showed ANI and dDDH values below 94.78 % and 58.8 % concerning type strains of the B. cepacia complex (Bcc) species. The phylogenetic maxi-mum likelihood tree constructed based on the multilocus sequence analysis of core genes (cMLSA) clustered the strains CCRMBC16, CCRMBC33, CCRMBC74, and CCRMBC171 and the strain CCRMBC51 in two exclusive clades, which did not cluster with any known species of the Bcc. Therefore, combined data from TYGS, ANI, dDDH, and cMLSA demonstrated that the strains represent two novel species of the Bcc, which we classified as Burkholderia semiarida sp. nov. and Burkholderia sola sp. nov., and proposed the strains CCRMBC74T (=IBSBF 3371 T = CBAS 905 T) and CCRMBC51T (=IBSBF3370T = CBAS 904 T) as type strains, respectively.
英文关键词	Allium cepa Burkholderia semiarida sp nov Burkholderia sola sp Novel species Onion diseases Taxogenomic approach
类型	Article
语种	英语
收录类别	SCI-E
WOS记录号	WOS:000957319300001
WOS关键词	MULTIPLE SEQUENCE ALIGNMENT ; DNA-DNA HYBRIDIZATION ; IDENTIFICATION ; MULTIVORANS ; GENOMOVARS ; ALGORITHM ; TOOL
WOS类目	Biotechnology & Applied Microbiology ; Microbiology
WOS研究方向	Biotechnology & Applied Microbiology ; Microbiology
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/398881
推荐引用方式 GB/T 7714	Velez, Leandro S.,Aburjaile, Flavia F.,Farias, Antonio R. G.,et al. Burkholderia semiarida sp. nov. and Burkholderia sola sp. nov., two novel B. cepacia complex species causing onion sour skin[J],2023,46(3).
APA	Velez, Leandro S..,Aburjaile, Flavia F..,Farias, Antonio R. G..,Baia, Ana D. B..,Oliveira, Willams J..,...&Gama, Marco A. S..(2023).Burkholderia semiarida sp. nov. and Burkholderia sola sp. nov., two novel B. cepacia complex species causing onion sour skin.SYSTEMATIC AND APPLIED MICROBIOLOGY,46(3).
MLA	Velez, Leandro S.,et al."Burkholderia semiarida sp. nov. and Burkholderia sola sp. nov., two novel B. cepacia complex species causing onion sour skin".SYSTEMATIC AND APPLIED MICROBIOLOGY 46.3(2023).