干旱区生态环境知识资源中心(Arid): In Vitro Seed and Clonal Propagation of the Mediterranean Bee Friendly Plant Anthyllis hermanniae L.

Arid

DOI	10.3390/su15054025
	In Vitro Seed and Clonal Propagation of the Mediterranean Bee Friendly Plant Anthyllis hermanniae L.
	Martini, Aikaterini N. N.; Papafotiou, Maria
通讯作者	Martini, AN
来源期刊	SUSTAINABILITY
EISSN	2071-1050
出版年	2023
卷号	15 期号:5
英文摘要	Anthyllis hermanniae L. (Fabaceae) is a perennial Mediterranean shrub with the potential to be used as a bee-friendly ornamental plant in arid and semi-arid regions, valued for its tolerance of barren soils, winds, and strong temperature changes. With the aim of facilitating the introduction of the species into the horticulture industry, its in vitro seed and clonal propagation was investigated for the first time, to our knowledge. Seeds stored in the dark at room temperature for 4, 7, 9, 12, and 18 months germinated at percentages higher than 80% after scarification, when incubated in vitro in solid half-strength Murashige and Skoog (MS) medium at temperatures from 10 to 25 degrees C, while photoperiod (continuous darkness or 16 h light period/8 h dark) during incubation did not affect germination. Explants excised from in vitro grown seedlings established at higher percentages compared to explants from adult native plants, more efficiently in MS medium with 1.0 mg L-1 6 N benzyladenine (BA). During subcultures in the same medium, juvenile explants formed more and longer shoots than adult ones. Almost all adult explants formed shoots when subcultured in MS medium with 0.0 to 4.0 mg L-1 BA, zeatin, kinetin or 6-(gamma,gamma-dimethylallylamino)purine (2iP). Beta A at 0.5 to 2.0 mg L-1 induced many more shoots (17-21) per explant and much higher multiplication indices compared to all other cytokinins, while longer shoots were produced in a medium without hormones or with 0.5-1.0 mg L-1 2iP. Microshoots cultured in half-strength MS medium with 0.0-4.0 mg L-1 indole-3-butyric acid rooted at highest percentage (around 70%) in the medium containing 4.0 mg L-1 IBA, while microshoots of juvenile origin developed more and longer roots compared to adult ones. Micropropagated plantlets were successfully acclimatized ex vitro (>97%), regardless of their origin. The efficient micropropagation of A. hermaniae will facilitate its sustainable exploitation as a bee-friendly landscape plant, a forage plant for honeybees in Mediterranean areas, and a medicinal plant.
英文关键词	acclimatization auxin cytokinin explant origin germination conditions micropropagation native xerophytic ornamental proliferation rooting seed pretreatment
类型	Article
语种	英语
开放获取类型	gold
收录类别	SCI-E ; SSCI
WOS记录号	WOS:000947859600001
WOS关键词	ENVIRONMENTAL-FACTORS ; MICROPROPAGATION ; GERMINATION ; ECOPHYSIOLOGY ; GERMINABILITY ; CYTISOIDES ; JUVENILE ; DORMANCY ; GROWTH ; SIZE
WOS类目	Green & Sustainable Science & Technology ; Environmental Sciences ; Environmental Studies
WOS研究方向	Science & Technology - Other Topics ; Environmental Sciences & Ecology
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/398769
推荐引用方式 GB/T 7714	Martini, Aikaterini N. N.,Papafotiou, Maria. In Vitro Seed and Clonal Propagation of the Mediterranean Bee Friendly Plant Anthyllis hermanniae L.[J],2023,15(5).
APA	Martini, Aikaterini N. N.,&Papafotiou, Maria.(2023).In Vitro Seed and Clonal Propagation of the Mediterranean Bee Friendly Plant Anthyllis hermanniae L..SUSTAINABILITY,15(5).
MLA	Martini, Aikaterini N. N.,et al."In Vitro Seed and Clonal Propagation of the Mediterranean Bee Friendly Plant Anthyllis hermanniae L.".SUSTAINABILITY 15.5(2023).