干旱区生态环境知识资源中心(Arid): Transcriptome analysis revealed water balance controlled by ZxOSCA1.1 and lateral root development are crucial strategies for Zygophyllum xanthoxylon

Arid

DOI	10.1016/j.sajb.2023.07.010
	Transcriptome analysis revealed water balance controlled by ZxOSCA1.1 and lateral root development are crucial strategies for Zygophyllum xanthoxylon
	Fu, Gaofei; Ma, Yulu; Li, Lin; Wang, Yue; Wu, Shengdan; Guo, Haiyang; Zhang, Linjing
通讯作者	Zhang, LJ
来源期刊	SOUTH AFRICAN JOURNAL OF BOTANY
ISSN	0254-6299
EISSN	1727-9321
出版年	2023
卷号	160 页码:354-368
英文摘要	The plant species that inhabit severe arid regions, such as xerophytes, are valuable genetic resources for deciphering water stress response mechanisms and breeding or engineering drought-resistant crop varieties. Zygophyllum xanthoxylon (Zygophyllaceae), succulent perennial xerophyte, is extensively distributed in desert and semi-desert regions in northwestern China and gets little attention. Herein, we used high-throughput transcriptome sequencing to profile gene expression at the whole-blade tissue level after drought treatment. Differential expression gene analysis and weighted gene co-expression network analysis (WGCNA) identified a diversity of drought-responsive genes encoding ion channels or exchanger proteins, such as outward rectifying K+ channel 1 (KCO1), involving lateral root (LR) formation, including NAC1, RAPID ALKALINIZATION FACTOR-LIKE 34 (RALFL34), expansion-like B1 (EXLB1), EXPANSIN 2 (EXPA2) and pectin methylesterase 3 (PME3). We hypothesize that a regulatory network is responsible for the LR development in Z. xanthoxylon. Besides, hyperosmolality-gated calcium-permeable channels (OSCA) genes, such as ZxOSCA1.1, were induced by polyethylene glycol (PEG-6000) treatment. To find out whether ZxOSCA1.1 plays a role in response to the water stress like the other species, we performed functional validation of ZxOSCA1.1 by transiently knocking down using virus-induced gene silencing (VIGS). The results revealed that ZxOSCA1.1 did affect the stomatal movement, water loss, and chlorophyll content. Our research demonstrates that osmotic perception controlled by upstream OSCA1.1 and the lateral root formation are important self-protection strategy for Z. xanthoxylon. Also, this study provides more perspectives insight into the molecular mechanisms underlying drought stress and aids for engineering drought-resistant crops.
英文关键词	ZxOSCA1 1 Zygophyllum xanthoxylon ZxOSCA3 VIGS Stomatal conductance Lateral root formation
类型	Article
语种	英语
收录类别	SCI-E
WOS记录号	WOS:001046946300001
WOS关键词	VIRUS-INDUCED GENE ; SIGNAL-TRANSDUCTION ; EXPRESSION ANALYSIS ; STRESS TOLERANCE ; PROLINE CONTENT ; PLANT-GROWTH ; CELL-DEATH ; ARABIDOPSIS ; DROUGHT ; CALCIUM
WOS类目	Plant Sciences
WOS研究方向	Plant Sciences
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/398697
推荐引用方式 GB/T 7714	Fu, Gaofei,Ma, Yulu,Li, Lin,et al. Transcriptome analysis revealed water balance controlled by ZxOSCA1.1 and lateral root development are crucial strategies for Zygophyllum xanthoxylon[J],2023,160:354-368.
APA	Fu, Gaofei.,Ma, Yulu.,Li, Lin.,Wang, Yue.,Wu, Shengdan.,...&Zhang, Linjing.(2023).Transcriptome analysis revealed water balance controlled by ZxOSCA1.1 and lateral root development are crucial strategies for Zygophyllum xanthoxylon.SOUTH AFRICAN JOURNAL OF BOTANY,160,354-368.
MLA	Fu, Gaofei,et al."Transcriptome analysis revealed water balance controlled by ZxOSCA1.1 and lateral root development are crucial strategies for Zygophyllum xanthoxylon".SOUTH AFRICAN JOURNAL OF BOTANY 160(2023):354-368.