干旱区生态环境知识资源中心(Arid): Antimicrobial potential of the endophytic actinobacteria isolated from Harpagophytum procumbens: A Southern African medicinal plant

Arid

DOI	10.1016/j.sajb.2023.03.030
	Antimicrobial potential of the endophytic actinobacteria isolated from Harpagophytum procumbens: A Southern African medicinal plant
	Lang, Magdalena Elizabeth; Sibanda, Timothy; Louw, Stefan; Uzabakiriho, Jean Damascene
通讯作者	Uzabakiriho, JD
来源期刊	SOUTH AFRICAN JOURNAL OF BOTANY
ISSN	0254-6299
EISSN	1727-9321
出版年	2023
卷号	156 页码:268-277
英文摘要	Endophytic actinobacteria associated with ethnobotanical medicinal plants are key reservoirs for the discovery of novel biomolecules and enzyme activities. The knowledge of such endophytic actinobacteria in the Southern Africa arid regions remains scanty. This study was designed to isolate and investigate the antimicrobial activity and determine the taxonomic diversity of endophytic actinobacteria from the Devil's claw (Harpagophytum procumbens), a well-known Kalahari Desert plant with indigenous pharmaceutical uses. The antimicrobial activity was evaluated using perpendicular screening, culture filtrate extracts disk diffusion and minimum inhibitory concentration (MIC) methods. Amongst the various extracts (ethyl acetate, chloroform and methanol) tested, ethyl acetate disc diffusion fraction exhibited a broad-spectrum antimicrobial activity against test organisms and significantly higher zone of inhibition and lower MIC values (p = 0.000) as compared to other extracts. Further, greater inhibitory activities against gram-negative than gram-positive bacteria were observed. Antimicrobial analysis coupled with the results of amplifying gene clusters coding for polyketide synthetase I (PKS-I) and nonribosomal peptide synthetase (NRPS) demonstrated a significant broad-spectrum potential against all the test organisms except C. albicans. The subsequent 16S rRNA gene partial sequence analysis of the 23 isolates revealed 6 families affiliated to 7 genera that included the dominant Streptomyces genus (53%) whereas rare genera (Agromyces, Nocardiopsis, Rubrobacter, Patulibacter, Rhodococcus, Curtobacterium) and 3 unidentified strains accounted for 43%. A total of 23 volatile compounds were identified in the three extracts using gas chromatography - mass spectrometry (GC-MS). Extracts B12, B44 and A65 contained 10, 9 and 14 compounds, respectively. These volatile compounds include long chain alkanes, alkenes/alcohols, esters and amides. This study suggests that the endophytic actinobacteria isolated from H. procumbens are diverse and promising sources of bioactive antimicrobial compounds.(c) 2023 Published by Elsevier B.V. on behalf of SAAB.
英文关键词	Harpagophytum procumbens Actinobacteria Antimicrobial Endophytes Medicinal plants GC-MS
类型	Article
语种	英语
收录类别	SCI-E
WOS记录号	WOS:000961198200001
WOS关键词	SP NOV. ; EMENDED DESCRIPTION ; RHIZOSPHERE ; DIVERSITY ; IDENTIFICATION ; SOIL
WOS类目	Plant Sciences
WOS研究方向	Plant Sciences
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/398692
推荐引用方式 GB/T 7714	Lang, Magdalena Elizabeth,Sibanda, Timothy,Louw, Stefan,et al. Antimicrobial potential of the endophytic actinobacteria isolated from Harpagophytum procumbens: A Southern African medicinal plant[J],2023,156:268-277.
APA	Lang, Magdalena Elizabeth,Sibanda, Timothy,Louw, Stefan,&Uzabakiriho, Jean Damascene.(2023).Antimicrobial potential of the endophytic actinobacteria isolated from Harpagophytum procumbens: A Southern African medicinal plant.SOUTH AFRICAN JOURNAL OF BOTANY,156,268-277.
MLA	Lang, Magdalena Elizabeth,et al."Antimicrobial potential of the endophytic actinobacteria isolated from Harpagophytum procumbens: A Southern African medicinal plant".SOUTH AFRICAN JOURNAL OF BOTANY 156(2023):268-277.