干旱区生态环境知识资源中心(Arid): Metabolomic profile of seminal plasma from Guzera bulls (Bos indicus) with contrasting sperm freezability phenotypes

Arid

DOI	10.1111/rda.14453
	Metabolomic profile of seminal plasma from Guzera bulls (Bos indicus) with contrasting sperm freezability phenotypes
	Pessoa, Eduardo Rodrigues; Vasconcelos, Fabio Roger; Paula-Marinho, Stelamaris de Oliveira; Daloso, Danilo de Menezes; Guerreiro, Denise Damasceno; Martins, Jorge Andre Matias; Gomes-Filho, Eneas; Moura, Arlindo Alencar
通讯作者	Moura, AA
来源期刊	REPRODUCTION IN DOMESTIC ANIMALS
ISSN	0936-6768
EISSN	1439-0531
出版年	2023
卷号	58 期号:10 页码:1379-1392
英文摘要	The present study evaluated the seminal plasma metabolome of Bos indicus Guzera bulls with good (n = 4) and poor (n = 5) sperm freezability. Animals were raised in natural pasture of a 'Caatinga' ecosystem, in the semi-arid region of Brazil. Seminal plasma samples were subjected to gas chromatography coupled to mass spectrometry and data, analysed using bioinformatics tools (Cytoscape with the MetScape plug-in). Sixty-two metabolites were identified in the bovine seminal plasma. Fatty acids and conjugates and organic compounds were the predominant seminal fluid metabolites, followed by carboxylic acids and derivatives, amino acids, benzenes and steroids and derivatives, carbohydrates and carbohydrate conjugates and prenol lipids. Multivariate analysis indicated a distinct separation of seminal plasma metabolomes from bulls with contrasting sperm freezability. Abundances of propanoic acid, d-ribose and glycine were greater in the seminal plasma of bulls with good sperm freezability. Heptadecanoic acid and undecanoic acid were the predominant in bulls of poor sperm freezability. Propanoic acid is an energy source for spermatozoa and may act as an antimicrobial component in semen. Glycine acts against oxidizing and denaturing reactions. d-ribose is also an energy source and reduces apoptosis and oxidative stress. Undecanoic acid may protect sperm against fungal damage. This study provides fundamental information approximately the seminal plasma metabolome of tropically adapted bulls and its association with sperm freezability. However, further studies with larger groups of animals are needed to validate those metabolites as markers of sperm freezability. This strategy could support the selection of sires with superior sperm cryoresistance.
英文关键词	d-ribose mass spectrometry metabolites propanoic acid sperm cryoresistance
类型	Article
语种	英语
开放获取类型	Bronze
收录类别	SCI-E
WOS记录号	WOS:001050293700001
WOS关键词	CHAIN FATTY-ACIDS ; LIPID-PEROXIDATION ; EJACULATED SPERM ; PROPIONIC-ACID ; MAJOR PROTEINS ; AMINO-ACIDS ; CRYOPRESERVATION ; SPERMATOZOA ; FERTILITY ; MOTILITY
WOS类目	Agriculture, Dairy & Animal Science ; Reproductive Biology ; Veterinary Sciences
WOS研究方向	Agriculture ; Reproductive Biology ; Veterinary Sciences
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/398364
推荐引用方式 GB/T 7714	Pessoa, Eduardo Rodrigues,Vasconcelos, Fabio Roger,Paula-Marinho, Stelamaris de Oliveira,et al. Metabolomic profile of seminal plasma from Guzera bulls (Bos indicus) with contrasting sperm freezability phenotypes[J],2023,58(10):1379-1392.
APA	Pessoa, Eduardo Rodrigues.,Vasconcelos, Fabio Roger.,Paula-Marinho, Stelamaris de Oliveira.,Daloso, Danilo de Menezes.,Guerreiro, Denise Damasceno.,...&Moura, Arlindo Alencar.(2023).Metabolomic profile of seminal plasma from Guzera bulls (Bos indicus) with contrasting sperm freezability phenotypes.REPRODUCTION IN DOMESTIC ANIMALS,58(10),1379-1392.
MLA	Pessoa, Eduardo Rodrigues,et al."Metabolomic profile of seminal plasma from Guzera bulls (Bos indicus) with contrasting sperm freezability phenotypes".REPRODUCTION IN DOMESTIC ANIMALS 58.10(2023):1379-1392.