干旱区生态环境知识资源中心(Arid): Identification of novel smORFs and microprotein acting in response to rehydration of Nostoc flagelliforme

Arid

DOI	10.1002/pmic.202200473
	Identification of novel smORFs and microprotein acting in response to rehydration of Nostoc flagelliforme
	Peng, Zhao; Huwanixi, Aishuake; Wan, Cuihong
通讯作者	Wan, CH
来源期刊	PROTEOMICS
ISSN	1615-9853
EISSN	1615-9861
出版年	2023
卷号	23 期号:12
英文摘要	Nostoc flagelliforme, a terrestrial cyanobacterium spread throughout arid and semi-arid areas, has been long known for its outstanding adaptability to extremely dry conditions. This microorganism is able to recover biological activities within hours after months of anhydrobiosis state, attracting investigation through proteomic analysis. Except for canonical proteome, microproteins encoded by small ORFs (smORFs) have recently been regarded as indispensable participants in metabolic processes. However, the involvement of smORFs in N. flagelliforme remains unknown. Here we first constructed a smORF database in N. flagelliforme using bioinformatic prediction, resulting in 6072 novel smORFs. Then LS-MS/MS analysis was applied to identify expression patterns of microproteins and seek smORFs and their encoded microprotein playing a role during rehydration. In total, 18 novel microproteins were mined based on a smORF searching strategy combined with three proteomic assays, of which five were annotated as ribosomal proteins, one as RNA polymerase subunit, and one as acetohydroxy acid isomeroreductase. We also suggested the possible functions of smORFs according to their expression pattern and discovered two neighboring and homologous smORFs. All these results will expand our knowledge of smORFs-encoded microproteins and their relation to the stress response of extremophilic microorganisms.
英文关键词	cyanobacteria drought stress LC-MS MS SEP smORF
类型	Article
语种	英语
收录类别	SCI-E
WOS记录号	WOS:000961606000001
WOS关键词	OPEN READING FRAMES ; PHOTOSYNTHETIC RECOVERY ; DESICCATION-TOLERANCE ; MASS SPECTROMETRISTS ; COMPARATIVE GENOMICS ; SMALL PROTEINS ; SEARCH ; CYANOPHYCEAE ; CYANOBACTERIUM ; DEHYDRATION
WOS类目	Biochemical Research Methods ; Biochemistry & Molecular Biology
WOS研究方向	Biochemistry & Molecular Biology
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/398183
推荐引用方式 GB/T 7714	Peng, Zhao,Huwanixi, Aishuake,Wan, Cuihong. Identification of novel smORFs and microprotein acting in response to rehydration of Nostoc flagelliforme[J],2023,23(12).
APA	Peng, Zhao,Huwanixi, Aishuake,&Wan, Cuihong.(2023).Identification of novel smORFs and microprotein acting in response to rehydration of Nostoc flagelliforme.PROTEOMICS,23(12).
MLA	Peng, Zhao,et al."Identification of novel smORFs and microprotein acting in response to rehydration of Nostoc flagelliforme".PROTEOMICS 23.12(2023).