干旱区生态环境知识资源中心(Arid): Characterization of terminal flowering cowpea (Vigna unguiculata (L.) Walp.) mutants obtained by induced mutagenesis digs out the loss-of-function of phosphatidylethanolamine-binding protein

Arid

DOI	10.1371/journal.pone.0295509
	Characterization of terminal flowering cowpea (Vigna unguiculata (L.) Walp.) mutants obtained by induced mutagenesis digs out the loss-of-function of phosphatidylethanolamine-binding protein
	Eswaramoorthy, Vijayakumar; Kandasamy, Thangaraj; Thiyagarajan, Kalaimagal; Chockalingam, Vanniarajan; Jegadeesan, Souframanien; Natesan, Senthil; Adhimoolam, Karthikeyan; Prabhakaran, Jeyakumar; Singh, Ramji; Muthurajan, Raveendran
通讯作者	Thiyagarajan, K
来源期刊	PLOS ONE
ISSN	1932-6203
出版年	2023
卷号	18 期号:12
英文摘要	Cowpea (Vigna unguiculata (L.) Walp) is one of the major food legume crops grown extensively in arid and semi-arid regions of the world. The determinate habit of cowpea has many advantages over the indeterminate and is well adapted to modern farming systems. Mutation breeding is an active research area to develop the determinate habit of cowpea. The present study aimed to develop new determinate habit mutants with terminal flowering (TFL) in locally well-adapted genetic backgrounds. Consequently, the seeds of popular cowpea cv P152 were irradiated with doses of gamma rays (200, 250, and, 300 Gy), and the M-1 populations were grown. The M-2 populations were produced from the M-1 progenies and selected determinate mutants (TFLCM-1 and TFLCM-2) from the M-2 generation (200 Gy) were forwarded up to the M-5 generation to characterize the mutants and simultaneously they were crossed with P152 to develop a MutMap population. In the M5 generation, determinate mutants (80-81 days) were characterized by evaluating the TFL growth habit, longer peduncles (30.75-31.45 cm), erect pods (160 degrees- 200 degrees), number of pods per cluster (4-5 nos.), and early maturity. Further, sequencing analysis of the VuTFL1 gene in the determinate mutants and MutMap population revealed a single nucleotide transversion (A-T at 1196 bp) in the fourth exon and asparagine (N) to tyrosine (Y) amino acid change at the 143(rd) position of phosphatidylethanolamine-binding protein (PEBP). Notably, the loss of function PEPB with a higher confidence level modification of anti-parallel beta-sheets and destabilization of the protein secondary structure was observed in the mutant lines. Quantitative real-time PCR (qRT-PCR) analysis showed that the VuTFL1 gene was downregulated at the flowering stage in TFL mutants. Collectively, the insights garnered from this study affirm the effectiveness of induced mutation in modifying the plant's ideotype. The TFL mutants developed during this investigation have the potential to serve as a valuable resource for fostering determinate traits in future cowpea breeding programs and pave the way for mechanical harvesting.
类型	Article
语种	英语
开放获取类型	gold
收录类别	SCI-E
WOS记录号	WOS:001158744900047
WOS关键词	INFLORESCENCE ARCHITECTURE ; GENE FAMILY ; DETERMINATE ; TRANSITION ; INITIATION ; HOMOLOG ; YIELD ; TIME ; TFL1
WOS类目	Multidisciplinary Sciences
WOS研究方向	Science & Technology - Other Topics
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/398139
推荐引用方式 GB/T 7714	Eswaramoorthy, Vijayakumar,Kandasamy, Thangaraj,Thiyagarajan, Kalaimagal,et al. Characterization of terminal flowering cowpea (Vigna unguiculata (L.) Walp.) mutants obtained by induced mutagenesis digs out the loss-of-function of phosphatidylethanolamine-binding protein[J],2023,18(12).
APA	Eswaramoorthy, Vijayakumar.,Kandasamy, Thangaraj.,Thiyagarajan, Kalaimagal.,Chockalingam, Vanniarajan.,Jegadeesan, Souframanien.,...&Muthurajan, Raveendran.(2023).Characterization of terminal flowering cowpea (Vigna unguiculata (L.) Walp.) mutants obtained by induced mutagenesis digs out the loss-of-function of phosphatidylethanolamine-binding protein.PLOS ONE,18(12).
MLA	Eswaramoorthy, Vijayakumar,et al."Characterization of terminal flowering cowpea (Vigna unguiculata (L.) Walp.) mutants obtained by induced mutagenesis digs out the loss-of-function of phosphatidylethanolamine-binding protein".PLOS ONE 18.12(2023).