干旱区生态环境知识资源中心(Arid): Establishment of an Efficient and Rapid Regeneration System for a Rare Shrubby Desert Legume Eremosparton songoricum

Arid

DOI	10.3390/plants12203535
	Establishment of an Efficient and Rapid Regeneration System for a Rare Shrubby Desert Legume Eremosparton songoricum
	Qiao, Siqi; Jin, Pei; Liu, Xiaojie; Liang, Yuqing; Yang, Ruirui; Bai, Wenwan; Zhang, Daoyuan; Li, Xiaoshuang
通讯作者	Li, XS
来源期刊	PLANTS-BASEL
ISSN	2223-7747
出版年	2023
卷号	12 期号:20
英文摘要	Eremosparton songoricum (Litv.) Vass. is a rare and extremely drought-tolerant legume shrub that is distributed in Central Asia. E. songoricum naturally grows on bare sand and can tolerate multiple extreme environmental conditions. It is a valuable and important plant resource for desertification prevention and environmental protection, as well as a good material for the exploration of stress tolerance mechanisms and excellent tolerant gene mining. However, the regeneration system for E. songoricum has not yet been established, which markedly limits the conservation and utilization of this endangered and valuable desert legume. Assimilated branches derived from seedlings were cultured on several MS mediums supplemented with various concentrations of TDZ or 6-BA in different combinations with NAA. The results showed that the most efficient multiplication medium was MS medium supplemented with 0.4 mg/L 6-BA and 0.1 mg/L NAA. The most efficient rooting medium was WPM + 25 g/L sucrose. The highest survival rate (77.8%) of transplantation was achieved when the ratio of sand to vermiculite was 1:1. In addition, the optimal callus induction medium was MS + 30 g/L sucrose + 2 mg/L TDZ + 0.5 mg/L NAA in darkness. The E. songoricum callus treated with 100 mM NaCl and 300 mM mannitol on MS medium could be used in proper salt and drought stress treatments in subsequent gene function tests. A rapid and efficient regeneration system for E. songoricum that allowed regeneration within 3 months was developed. The protocol will contribute to the conservation and utilization of this rare and endangered desert stress-tolerant species and also provide a fundamental basis for gene functional analysis in E. songoricum.
英文关键词	desert legume tissue culture hormone concentration regeneration Eremosparton songoricum callus
类型	Article
语种	英语
开放获取类型	Green Published, gold
收录类别	SCI-E
WOS记录号	WOS:001099412000001
WOS关键词	IN-VITRO REGENERATION ; SOMATIC EMBRYOGENESIS ; PLANT-REGENERATION ; ORGANOGENESIS ; EXPLANTS ; MICROPROPAGATION ; PROPAGATION ; INDUCTION ; STRESS ; CALLUS
WOS类目	Plant Sciences
WOS研究方向	Plant Sciences
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/398100
推荐引用方式 GB/T 7714	Qiao, Siqi,Jin, Pei,Liu, Xiaojie,et al. Establishment of an Efficient and Rapid Regeneration System for a Rare Shrubby Desert Legume Eremosparton songoricum[J],2023,12(20).
APA	Qiao, Siqi.,Jin, Pei.,Liu, Xiaojie.,Liang, Yuqing.,Yang, Ruirui.,...&Li, Xiaoshuang.(2023).Establishment of an Efficient and Rapid Regeneration System for a Rare Shrubby Desert Legume Eremosparton songoricum.PLANTS-BASEL,12(20).
MLA	Qiao, Siqi,et al."Establishment of an Efficient and Rapid Regeneration System for a Rare Shrubby Desert Legume Eremosparton songoricum".PLANTS-BASEL 12.20(2023).