Arid
DOI10.3390/plants12152885
Multiple-Genome-Based Simple Sequence Repeat Is an Efficient and Successful Method in Genotyping and Classifying Different Jujube Germplasm Resources
Li, Bin; Muhammad, Noor; Zhang, Shufeng; Lan, Yunxin; Yang, Yihan; Han, Shoukun; Liu, Mengjun; Yang, Meng
通讯作者Yang, M
来源期刊PLANTS-BASEL
ISSN2223-7747
出版年2023
卷号12期号:15
英文摘要Jujube (Ziziphus jujuba Mill.) is a commercially important tree native to China, known for its high nutritional value and widespread distribution, as well as its diverse germplasm resources. Being resilient to harsh climatic conditions, the cultivation of jujube could provide a solution to food insecurity and income for people of arid and semi-arid regions in and outside of China. The evaluation of germplasm resources and genetic diversity in jujube necessitates the use of Simple Sequence Repeat (SSR) markers. SSR markers are highly polymorphic and can be used to evaluate the genetic diversity within and between cultivars of Chinese jujube, and are important for conservation biology, breeding programs, and the discovery of important traits for Chinese jujube improvement in China and abroad. However, traditional methods of SSR development are time-consuming and inadequate to meet the growing research demands. To address this issue, we developed a novel approach called Multiple-Genome-Based SSR identification (MGB-SSR), which utilizes the genomes of three jujube cultivars to rapidly screen for polymorphic SSRs in the jujube genome. Through the screening process, we identified 12 pairs of SSR primers, which were then used to successfully classify 249 jujube genotypes. Based on the genotyping results, a digital ID card was established, enabling the complete identification of all 249 jujube plants. The MGB-SSR approach proved efficient in rapidly detecting polymorphic SSRs within the jujube genome. Notably, this study represents the first successful differentiation of jujube germplasm resources using 12 SSR markers, with 4 markers successfully identifying triploid jujube genotypes. These findings offer valuable information for the classification of Chinese jujube germplasm, thereby providing significant assistance to jujube researchers and breeders in identifying unknown jujube germplasm.
英文关键词jujube Simple Sequence Repeat (SSR) multiple-genome-based SSR (MGB-SSR) genetic diversity digital ID card
类型Article
语种英语
开放获取类型gold, Green Published
收录类别SCI-E
WOS记录号WOS:001046331100001
WOS关键词GENETIC DIVERSITY ; MICROSATELLITE MARKERS ; POPULATION-STRUCTURE ; MOLECULAR IDENTITY ; SSR ; IDENTIFICATION ; ESTABLISHMENT ; PROGRAM ; DESIGN ; APPLE
WOS类目Plant Sciences
WOS研究方向Plant Sciences
资源类型期刊论文
条目标识符http://119.78.100.177/qdio/handle/2XILL650/398085
推荐引用方式
GB/T 7714
Li, Bin,Muhammad, Noor,Zhang, Shufeng,et al. Multiple-Genome-Based Simple Sequence Repeat Is an Efficient and Successful Method in Genotyping and Classifying Different Jujube Germplasm Resources[J],2023,12(15).
APA Li, Bin.,Muhammad, Noor.,Zhang, Shufeng.,Lan, Yunxin.,Yang, Yihan.,...&Yang, Meng.(2023).Multiple-Genome-Based Simple Sequence Repeat Is an Efficient and Successful Method in Genotyping and Classifying Different Jujube Germplasm Resources.PLANTS-BASEL,12(15).
MLA Li, Bin,et al."Multiple-Genome-Based Simple Sequence Repeat Is an Efficient and Successful Method in Genotyping and Classifying Different Jujube Germplasm Resources".PLANTS-BASEL 12.15(2023).
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