干旱区生态环境知识资源中心(Arid): Gene Coexpression Analysis Identifies Genes Associated with Chlorophyll Content and Relative Water Content in Pearl Millet

Arid

DOI	10.3390/plants12061412
	Gene Coexpression Analysis Identifies Genes Associated with Chlorophyll Content and Relative Water Content in Pearl Millet
	Shinde, Harshraj; Dudhate, Ambika; Sathe, Atul; Paserkar, Neha; Wagh, Sopan Ganpatrao; Kadam, Ulhas Sopanrao
通讯作者	Shinde, H
来源期刊	PLANTS-BASEL
ISSN	2223-7747
出版年	2023
卷号	12 期号:6
英文摘要	Pearl millet is a significant crop that is tolerant to abiotic stresses and is a staple food of arid regions. However, its underlying mechanisms of stress tolerance are not fully understood. Plant survival is regulated by the ability to perceive a stress signal and induce appropriate physiological changes. Here, we screened for genes regulating physiological changes such as chlorophyll content (CC) and relative water content (RWC) in response to abiotic stress by using weighted gene coexpression network analysis (WGCNA) and clustering changes in physiological traits, i.e., CC and RWC associated with gene expression. Genes' correlations with traits were defined in the form of modules, and different color names were used to denote a particular module. Modules are groups of genes with similar patterns of expression, which also tend to be functionally related and co-regulated. In WGCNA, the dark green module (7082 genes) showed a significant positive correlation with CC, and the black (1393 genes) module was negatively correlated with CC and RWC. Analysis of the module positively correlated with CC highlighted ribosome synthesis and plant hormone signaling as the most significant pathways. Potassium transporter 8 and monothiol glutaredoxin were reported as the topmost hub genes in the dark green module. In Clust analysis, 2987 genes were found to display a correlation with increasing CC and RWC. Furthermore, the pathway analysis of these clusters identified the ribosome and thermogenesis as positive regulators of RWC and CC, respectively. Our study provides novel insights into the molecular mechanisms regulating CC and RWC in pearl millet.
英文关键词	gene expression WGCNA gene clustering module pathways pearl millet relative water content
类型	Article
语种	英语
开放获取类型	Green Submitted, gold, Green Published
收录类别	SCI-E
WOS记录号	WOS:000958131200001
WOS关键词	STRESS ; PLANTS
WOS类目	Plant Sciences
WOS研究方向	Plant Sciences
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/398057
推荐引用方式 GB/T 7714	Shinde, Harshraj,Dudhate, Ambika,Sathe, Atul,et al. Gene Coexpression Analysis Identifies Genes Associated with Chlorophyll Content and Relative Water Content in Pearl Millet[J],2023,12(6).
APA	Shinde, Harshraj,Dudhate, Ambika,Sathe, Atul,Paserkar, Neha,Wagh, Sopan Ganpatrao,&Kadam, Ulhas Sopanrao.(2023).Gene Coexpression Analysis Identifies Genes Associated with Chlorophyll Content and Relative Water Content in Pearl Millet.PLANTS-BASEL,12(6).
MLA	Shinde, Harshraj,et al."Gene Coexpression Analysis Identifies Genes Associated with Chlorophyll Content and Relative Water Content in Pearl Millet".PLANTS-BASEL 12.6(2023).