干旱区生态环境知识资源中心(Arid): Novel inducible promoter DREB1G cloned from date palm exhibits high fold expression over AtRD29 to drought and salinity stress

Arid

DOI	10.1007/s11240-023-02460-3
	Novel inducible promoter DREB1G cloned from date palm exhibits high fold expression over AtRD29 to drought and salinity stress
	Kodackattumannil, Preshobha; Whitley, Kenna; Sasi, Shina; Lekshmi, Geetha; Krishnan, Saranya; Al Senaani, Salima; Kottackal, Martin; Amiri, Khaled M. A.
通讯作者	Kottackal, M ; Amiri, KMA
来源期刊	PLANT CELL TISSUE AND ORGAN CULTURE
ISSN	0167-6857
EISSN	1573-5044
出版年	2023
卷号	154 期号:2 页码:367-380
英文摘要	Right and timely expression of the stress regulatory genes is required for plants to compete against abiotic stresses; it necessitates the isolation and characterization of stress-responsive promoters for improving crops' tolerance to abiotic stresses. Dehydration Responsive Element Binding (DREB) regulates the expression of numerous stress-responsive genes in plants and leads an inevitable role in the adaptation of plants to abiotic stresses. In this study, the promoter region of Phoenix dactylifera (Date palm, a major fruit crop of the arid region) PdDREB1G gene was isolated and characterized for the first time. A comparison of the activity of two promoter fragments, 880 bp (DS) and 1.6 kb (DF) of PdDREB1G to AtRD29A was performed. Histochemical assay displayed remarkable GUS staining and RT-qPCR analysis confirmed the induction of GUS expression in T-3 plants of transformed tobacco subjected to different abiotic stresses. Furthermore, compared with the widely used AtRD29A promoter, the relative expression of GUS in leaves by DS and DF was three and twofold higher under salt stress, respectively, while it was twofold in polyethylene glycol (PEG) and abscisic acid (ABA) for DS. Under SA stress, DF and DS displayed 1.5 and onefold expression in leaves, respectively. In the root, DS showed a fourfold increased expression in salt, threefold in PEG and ABA, and twofold in SA. Hence, the DS promoter characterized in the present study becomes a choice over RD29A for abiotic stress responses and is useful to develop stress-tolerant transgenic plants by inducing the expression of stress-inducible genes on stress. Key messageNovel inducible promoter DREB1G cloned from the Date palm, a major fruit crop of the arid region, exhibiting high fold expression over AtRD29A to drought and salinity stress is useful to develop stress-tolerant transgenic plants by inducing the expression of stress-inducible genes on stress.
英文关键词	Abiotic stress GUS PdDREB1G Promoter Salinity Stress-inducible
类型	Article
语种	英语
开放获取类型	hybrid
收录类别	SCI-E
WOS记录号	WOS:000932735400001
WOS关键词	GENE-EXPRESSION ; LOW-TEMPERATURE ; FUNCTIONAL-CHARACTERIZATION ; CAMV-35S PROMOTER ; PLANT ; TOLERANCE ; ELEMENTS ; TOBACCO ; DEHYDRATION ; RESPONSES
WOS类目	Biotechnology & Applied Microbiology ; Plant Sciences
WOS研究方向	Biotechnology & Applied Microbiology ; Plant Sciences
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/398029
推荐引用方式 GB/T 7714	Kodackattumannil, Preshobha,Whitley, Kenna,Sasi, Shina,et al. Novel inducible promoter DREB1G cloned from date palm exhibits high fold expression over AtRD29 to drought and salinity stress[J],2023,154(2):367-380.
APA	Kodackattumannil, Preshobha.,Whitley, Kenna.,Sasi, Shina.,Lekshmi, Geetha.,Krishnan, Saranya.,...&Amiri, Khaled M. A..(2023).Novel inducible promoter DREB1G cloned from date palm exhibits high fold expression over AtRD29 to drought and salinity stress.PLANT CELL TISSUE AND ORGAN CULTURE,154(2),367-380.
MLA	Kodackattumannil, Preshobha,et al."Novel inducible promoter DREB1G cloned from date palm exhibits high fold expression over AtRD29 to drought and salinity stress".PLANT CELL TISSUE AND ORGAN CULTURE 154.2(2023):367-380.