干旱区生态环境知识资源中心(Arid): The transcription factor CREB3-2 regulated neutral lipase gene expression in ovary of Nilaparvata lugens

Arid

DOI	10.1016/j.pestbp.2023.105632
	The transcription factor CREB3-2 regulated neutral lipase gene expression in ovary of Nilaparvata lugens
	Lin, Xumin; Zhang, Huihui; Gao, Haoli; Yuan, Xiaowei; Liu, Zewen
通讯作者	Liu, ZW
来源期刊	PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
ISSN	0048-3575
EISSN	1095-9939
出版年	2023
卷号	196
英文摘要	The cyclic AMP-responsive element-binding protein 3 (CREB3) members have unique regulatory roles in cellular lipid metabolism as transcription factors. Two CREB3 proteins in Nilaparvata lugens were identified and analyzed. In ovary, when silencing NlCREB3-2, triacylglycerol (TAG) content dramatically increased but glycerol and free fatty acid (FFA) significantly decreased, which implicated that NlCREB3-2 was involved in the lipase-related TAG metabolism. In N. lugens, five neutral lipases with complete features for TAG hydrolytic activity and high expression in ovary were focused. Among them, the expression levels of three neutral lipase genes were significantly down-regulated by NlCREB3-2 RNAi. The direct regulation of NlCREB3-2 towards the three neutral lipase genes was evidenced by the dual-luciferase reporter assay. After jointly silencing three neutral lipase genes, TAG and glycerol contents displayed similar changes as NlCREB3-2 RNAi. The study proved that NlCREB3-2 participated in TAG metabolism in ovary via the direct activation towards the ovary-specific neutral lipase genes.
英文关键词	Nilaparvata lugens CREB3-2 Triacylglycerol metabolism Neutral lipase Ovary
类型	Article
语种	英语
收录类别	SCI-E
WOS记录号	WOS:001102833300001
WOS关键词	CREB/ATF-FAMILY ; BROWN PLANTHOPPER ; STRESS-RESPONSE ; SALIVARY-GLAND ; FAT-BODY ; DCREB-A ; DROSOPHILA ; ELEMENT ; INSECT ; OASIS
WOS类目	Biochemistry & Molecular Biology ; Entomology ; Physiology
WOS研究方向	Biochemistry & Molecular Biology ; Entomology ; Physiology
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/397992
推荐引用方式 GB/T 7714	Lin, Xumin,Zhang, Huihui,Gao, Haoli,et al. The transcription factor CREB3-2 regulated neutral lipase gene expression in ovary of Nilaparvata lugens[J],2023,196.
APA	Lin, Xumin,Zhang, Huihui,Gao, Haoli,Yuan, Xiaowei,&Liu, Zewen.(2023).The transcription factor CREB3-2 regulated neutral lipase gene expression in ovary of Nilaparvata lugens.PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY,196.
MLA	Lin, Xumin,et al."The transcription factor CREB3-2 regulated neutral lipase gene expression in ovary of Nilaparvata lugens".PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY 196(2023).