干旱区生态环境知识资源中心(Arid): The Zoige pioneer plant Leymus secalinus has different endophytic bacterial community structures to adapt to environmental conditions

Arid

DOI	10.7717/peerj.15363
	The Zoige pioneer plant Leymus secalinus has different endophytic bacterial community structures to adapt to environmental conditions
	Xia, Yue; He, Ruipeng; Xu, Wanru; Zhang, Jie
通讯作者	Zhang, J
来源期刊	PEERJ
ISSN	2167-8359
出版年	2023
卷号	11
英文摘要	Background. Leymus secalinus is a pioneer plant grown in the Zoige desertified alpine grassland and it is also one of the dominant plant species used for environmental remediation. L. secalinus plays a large role in vegetation reconstruction in sandy land, but the abundance and diversity of its endophytes have not yet been investigated.Objectives. This study was performed to investigate the changes in the endophytic bacterial community structure of L. secalinus under different ecological environments and to analyze the effects of environmental changes and different plant tissues on the L. secalinus endophytic bacteria.Methods. Leaf, stem, and root tissue samples of L. secalinus were collected from Zoige Glassland (Alpine sandy land) and an open field nursery (Control). DNA was extracted and the 16S ribosomal DNA was amplified. The sequence library was sequenced on an Illumina MiSeq platform and clustered by operational taxonomic units (OTUs). alpha- diversity and beta-diversity analyses, species diversity analyses, functional prediction, and redundancy (RDA) analyses for the soil physicochemical properties were conducted.Results. alpha-diversity and beta-diversity analyses showed that the endophytic bacteria in L. secalinus varied in different areas and tissues. The abundance of Allorhizobium-Neorhizobium-Pararhizobium-Rhizobium, which is related to nitrogen fixation, in-creased significantly in the L. secalinus found in the Zoige Grassland. Moreover, the abundance of nutrition metabolism and anti-stress abilities increased in functional prediction in the desert samples. The soil physicochemical properties had an insignificant influence on bacterial diversity.Conclusion. The changes in the endophytic bacterial community structure in L. secalinus were significant and were caused by environmental alterations and plant choice. The endophytic bacteria in L. secalinus grown in alpine sandy land may have greater anti-stress properties and the ability to fix nitrogen, which has potential value in environmental remediation and agricultural production.
英文关键词	Leymus secalinus Endophytic bacterium Zoige alpine grassland Vegetation remediation Nitrogen fixation 16S rRNA gene sequencing
类型	Article
语种	英语
开放获取类型	Green Published, gold
收录类别	SCI-E
WOS记录号	WOS:000996335400004
WOS关键词	AEOLIAN DESERTIFICATION ; NITROGEN-FIXATION ; DIVERSITY ; GROWTH ; ROOTS ; CULTURES ; PLATEAU ; BASIN
WOS类目	Multidisciplinary Sciences
WOS研究方向	Science & Technology - Other Topics
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/397982
推荐引用方式 GB/T 7714	Xia, Yue,He, Ruipeng,Xu, Wanru,et al. The Zoige pioneer plant Leymus secalinus has different endophytic bacterial community structures to adapt to environmental conditions[J],2023,11.
APA	Xia, Yue,He, Ruipeng,Xu, Wanru,&Zhang, Jie.(2023).The Zoige pioneer plant Leymus secalinus has different endophytic bacterial community structures to adapt to environmental conditions.PEERJ,11.
MLA	Xia, Yue,et al."The Zoige pioneer plant Leymus secalinus has different endophytic bacterial community structures to adapt to environmental conditions".PEERJ 11(2023).