干旱区生态环境知识资源中心(Arid): Plant DNA barcode library for native flowering plants in the arid region of northwestern China

Arid

DOI	10.1111/1755-0998.13797
	Plant DNA barcode library for native flowering plants in the arid region of northwestern China
	Song, Feng; Li, Ting; Yan, Hai-Fei; Feng, Ying; Jin, Lu; Burgess, Kevin S. S.; Ge, Xue-Jun
通讯作者	Ge, XJ
来源期刊	MOLECULAR ECOLOGY RESOURCES
ISSN	1755-098X
EISSN	1755-0998
出版年	2023
卷号	23 期号:6 页码:1389-1402
英文摘要	DNA barcoding is a well-established tool for rapid species identification and biodiversity monitoring. A reliable and traceable DNA barcode reference library with extensive coverage is necessary but unavailable for many geographical regions. The arid region in northwestern China, a vast area of about 2.5 million km(2), is ecologically fragile and often overlooked in biodiversity studies. In particular, DNA barcode data from the arid region in China are lacking. We develop and evaluate the efficacy of an extensive DNA barcode library for native flowering plants in the arid region of northwestern China. Plant specimens were collected, identified and vouchered for this purpose. The database utilized four DNA barcode markers, namely rbcL, matK, ITS and ITS2, for 1816 accessions (representing 890 species from 385 genera and 72 families), and consisted of 5196 barcode sequences. Individual barcodes varied in resolution rates: species- and genus-level rates for rbcL, matK, ITS and ITS2 were 79.9%-51.1%/76.1%, 79.9%-67.2%/88.9%, 85.0%-72.0%/88.2% and 81.0%-67.4%/84.9%, respectively. The three-barcode combination of rbcL + matK + ITS (RMI) revealed a higher species- and genus-level resolution (75.5%/92.1%, respectively). A total of 110 plastomes were newly generated as super-barcodes to increase species resolution for seven species-rich genera, namely Astragalus, Caragana, Lactuca, Lappula, Lepidium, Silene and Zygophyllum. Plastomes revealed higher species resolution compared to standard DNA barcodes and their combination. We suggest future databases include super-barcodes, especially for species-rich and complex genera. The plant DNA barcode library in the current study provides a valuable resource for future biological investigations in the arid regions of China.
英文关键词	arid region DNA barcodes flowering plants reference library super-barcodes
类型	Article
语种	英语
收录类别	SCI-E
WOS记录号	WOS:000974453400001
WOS关键词	PHYLOGENETIC ANALYSIS ; CARAGANA-MICROPHYLLA ; CHLOROPLAST GENOMES ; C-KORSHINSKII ; NUCLEAR ; EVOLUTION ; INSIGHTS ; TOOL ; DIVERSIFICATION ; HYBRIDIZATION
WOS类目	Biochemistry & Molecular Biology ; Ecology ; Evolutionary Biology
WOS研究方向	Biochemistry & Molecular Biology ; Environmental Sciences & Ecology ; Evolutionary Biology
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/397867
推荐引用方式 GB/T 7714	Song, Feng,Li, Ting,Yan, Hai-Fei,et al. Plant DNA barcode library for native flowering plants in the arid region of northwestern China[J],2023,23(6):1389-1402.
APA	Song, Feng.,Li, Ting.,Yan, Hai-Fei.,Feng, Ying.,Jin, Lu.,...&Ge, Xue-Jun.(2023).Plant DNA barcode library for native flowering plants in the arid region of northwestern China.MOLECULAR ECOLOGY RESOURCES,23(6),1389-1402.
MLA	Song, Feng,et al."Plant DNA barcode library for native flowering plants in the arid region of northwestern China".MOLECULAR ECOLOGY RESOURCES 23.6(2023):1389-1402.