干旱区生态环境知识资源中心(Arid): Understanding the Effect of Different Glucose Concentrations in the Oligotrophic Bacterium Bacillus subtilis BS-G1 through Transcriptomics Analysis

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DOI	10.3390/microorganisms11102401
	Understanding the Effect of Different Glucose Concentrations in the Oligotrophic Bacterium Bacillus subtilis BS-G1 through Transcriptomics Analysis
	Chen, Liping; Wang, Chenglong; Su, Jianyu
通讯作者	Su, JY
来源期刊	MICROORGANISMS
EISSN	2076-2607
出版年	2023
卷号	11 期号:10
英文摘要	Glucose is an important carbon source for microbial growth, and its content in infertile soils is essential for the growth of bacteria. Since the mechanism of oligotrophic bacterium adaptation in barren soils is unclear, this research employed RNA-seq technology to examine the impact of glucose concentration on the oligotrophic bacterium B. subtilis BS-G1 in soil affected by desertification. A global transcriptome analysis (RNA-Seq) revealed that the significantly differentially expressed genes (DEGs) histidine metabolism, glutamate synthesis, the HIF-1 signaling pathway, sporulation, and the TCA cycle pathway of B. subtilis BS-G1 were significantly enriched with a 0.015 g/L glucose concentration (L group), compared to a 10 g/L glucose concentration (H group). The DEGs amino acid system, two-component system, metal ion transport, and nitrogen metabolism system of B. subtilis BS-G1 were significantly enriched in the 5 g/L glucose concentration (M group), compared with the H group. In addition, the present study identified the regulation pattern and key genes under a low-glucose environment (7 mRNAs and 16 sRNAs). This study primarily investigates the variances in the regulatory pathways of the oligotrophic B. subtilis BS-G1, which holds substantial importance in comprehending the mechanism underlying the limited sugar tolerance of oligotrophic bacteria.
英文关键词	oligotrophic bacteria low glucose tolerance sRNA transcriptome
类型	Article
语种	英语
开放获取类型	Green Published, Green Submitted, gold
收录类别	SCI-E
WOS记录号	WOS:001092559500001
WOS关键词	SMALL RNAS ; METABOLISM ; NITROGEN ; DIVERSE ; GROWTH ; IDENTIFICATION ; PROTEIN ; CARBON ; GENES ; HFQ
WOS类目	Microbiology
WOS研究方向	Microbiology
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/397855
推荐引用方式 GB/T 7714	Chen, Liping,Wang, Chenglong,Su, Jianyu. Understanding the Effect of Different Glucose Concentrations in the Oligotrophic Bacterium Bacillus subtilis BS-G1 through Transcriptomics Analysis[J],2023,11(10).
APA	Chen, Liping,Wang, Chenglong,&Su, Jianyu.(2023).Understanding the Effect of Different Glucose Concentrations in the Oligotrophic Bacterium Bacillus subtilis BS-G1 through Transcriptomics Analysis.MICROORGANISMS,11(10).
MLA	Chen, Liping,et al."Understanding the Effect of Different Glucose Concentrations in the Oligotrophic Bacterium Bacillus subtilis BS-G1 through Transcriptomics Analysis".MICROORGANISMS 11.10(2023).