干旱区生态环境知识资源中心(Arid): Screening and Identification of a Streptomyces Strain with Quorum-Sensing Inhibitory Activity and Effect of the Crude Extracts on Virulence Factors of Pseudomonas aeruginosa

Arid

DOI	10.3390/microorganisms11082079
	Screening and Identification of a Streptomyces Strain with Quorum-Sensing Inhibitory Activity and Effect of the Crude Extracts on Virulence Factors of Pseudomonas aeruginosa
	Zhang, Zhidong; Sun, Yang; Yi, Yuanyang; Bai, Xiaoyu; Zhu, Liying; Zhu, Jing; Gu, Meiying; Zhu, Yanlei; Jiang, Ling
通讯作者	Jiang, L
来源期刊	MICROORGANISMS
EISSN	2076-2607
出版年	2023
卷号	11 期号:8
英文摘要	Quorum-sensing (QS) is involved in numerous physiological processes in bacteria, such as biofilm formation, sporulation, and virulence formation. Therefore, the search for new quorum-sensing inhibitors (QSI) is a promising strategy that opens up a new perspective for controlling QS-mediated bacterial pathogens. To explore new QSIs, a strain named Streptomyces sp. D67 with QS inhibitory activity was isolated from the soil of the arid zone around the Kumutag Desert in Xinjiang. Phylogenetic analyses demonstrated that strain D67 shared the highest similarity with Streptomyces ardesiacus NBRC 15402T (98.39%), which indicated it represented a potential novel species in the Streptomyces genus. The fermentation crude extracts of strain D67 can effectively reduce the violacein production produced by Chromobacterium violaceum CV026 and the swarming and swimming abilities of Pseudomonas aeruginosa. It also has significant inhibitory activity on the production of virulence factors such as biofilm, pyocyanin, and rhamnolipids of P. aeruginosa in a significant concentration-dependent manner, but not on protease activity. A total of 618 compounds were identified from the fermentation crude extracts of strain D67 by LC-MS, and 19 compounds with significant QS inhibitory activity were observed. Overall, the strain with QS inhibitory activity was screened from Kumutag Desert in Xinjiang for the first time, which provided a basis for further research and development of new QSI.
英文关键词	quorum-sensing inhibitors Actinomycete Pseudomonas aeruginosa virulence factors
类型	Article
语种	英语
开放获取类型	Green Published, gold
收录类别	SCI-E
WOS记录号	WOS:001056019700001
WOS关键词	BIOFILM FORMATION ; SYSTEMS
WOS类目	Microbiology
WOS研究方向	Microbiology
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/397852
推荐引用方式 GB/T 7714	Zhang, Zhidong,Sun, Yang,Yi, Yuanyang,et al. Screening and Identification of a Streptomyces Strain with Quorum-Sensing Inhibitory Activity and Effect of the Crude Extracts on Virulence Factors of Pseudomonas aeruginosa[J],2023,11(8).
APA	Zhang, Zhidong.,Sun, Yang.,Yi, Yuanyang.,Bai, Xiaoyu.,Zhu, Liying.,...&Jiang, Ling.(2023).Screening and Identification of a Streptomyces Strain with Quorum-Sensing Inhibitory Activity and Effect of the Crude Extracts on Virulence Factors of Pseudomonas aeruginosa.MICROORGANISMS,11(8).
MLA	Zhang, Zhidong,et al."Screening and Identification of a Streptomyces Strain with Quorum-Sensing Inhibitory Activity and Effect of the Crude Extracts on Virulence Factors of Pseudomonas aeruginosa".MICROORGANISMS 11.8(2023).