干旱区生态环境知识资源中心(Arid): Bacterial Community Composition and Isolation of Actinobacteria from the Soil of Flaming Mountain in Xinjiang, China

Arid

DOI	10.3390/microorganisms11020489
	Bacterial Community Composition and Isolation of Actinobacteria from the Soil of Flaming Mountain in Xinjiang, China
	He, Zixuan; Wang, Yuxian; Bai, Xiaoyu; Chu, Min; Yi, Yuanyang; Zhu, Jing; Gu, Meiying; Jiang, Ling; Zhang, Zhidong
通讯作者	Zhang, ZD
来源期刊	MICROORGANISMS
EISSN	2076-2607
出版年	2023
卷号	11 期号:2
英文摘要	In this work, bacterial community composition and actinobacteria resources were explored in extremely hot and hyper-arid areas of Flaming Mountain. This was achieved through a combination of PCR amplicon sequencing of bacterial 16S rRNA gene and cultivation-dependent isolation and characterization efforts. According to the high-throughput sequencing results and soil characteristics, 11 kinds of media were firstly designed to isolate actinobacteria, following the screening and identification of related strains. The results showed that a total of 2994 operational taxonomic units (OTUs) were obtained, involving 22 phyla, 77 orders and 121 genera. Among them, actinobacteria with the relative abundance of 8% ranked third, accounting for 33 genera and 47 species. A total of 132 strains distributed by eight families and 11 genera of actinobacteria were isolated from 11 media, of which six strains were potential new species. Furthermore, the functional characteristics of isolated strains were preliminarily evaluated. The results showed that the obtained strains generally had tolerance against heat, salt and alkali. Fifty-two strains had antibacterial activity, 69 strains could produce hydrolases, and 12.4% of the strains had quorum sensing inhibitory activity. The present study has laid a solid foundation for further understanding the bacterial diversity and exploiting actinobacteria resources in the Flaming Mountain area.
英文关键词	Flaming Mountain bacterial community composition actinobacteria isolation and identification function evaluation
类型	Article
语种	英语
开放获取类型	Green Published, gold
收录类别	SCI-E
WOS记录号	WOS:000941459100001
WOS关键词	ATACAMA DESERT ; SEQUENCE ; MICROORGANISMS ; DIVERSITY
WOS类目	Microbiology
WOS研究方向	Microbiology
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/397847
推荐引用方式 GB/T 7714	He, Zixuan,Wang, Yuxian,Bai, Xiaoyu,et al. Bacterial Community Composition and Isolation of Actinobacteria from the Soil of Flaming Mountain in Xinjiang, China[J],2023,11(2).
APA	He, Zixuan.,Wang, Yuxian.,Bai, Xiaoyu.,Chu, Min.,Yi, Yuanyang.,...&Zhang, Zhidong.(2023).Bacterial Community Composition and Isolation of Actinobacteria from the Soil of Flaming Mountain in Xinjiang, China.MICROORGANISMS,11(2).
MLA	He, Zixuan,et al."Bacterial Community Composition and Isolation of Actinobacteria from the Soil of Flaming Mountain in Xinjiang, China".MICROORGANISMS 11.2(2023).