干旱区生态环境知识资源中心(Arid): Active Sulfate-Reducing Bacterial Community in the Camel Gut

Arid

DOI	10.3390/microorganisms11020401
	Active Sulfate-Reducing Bacterial Community in the Camel Gut
	Karnachuk, Olga V.; Panova, Inna A.; Panov, Vasilii L.; Ikkert, Olga P.; Kadnikov, Vitaly V.; Rusanov, Igor I.; Avakyan, Marat R.; Glukhova, Lubov B.; Lukina, Anastasia P.; Rakitin, Anatolii V.; Begmatov, Shahjahon; Beletsky, Alexey V.; Pimenov, Nikolai V.; Ravin, Nikolai V.
通讯作者	Karnachuk, OV
来源期刊	MICROORGANISMS
EISSN	2076-2607
出版年	2023
卷号	11 期号:2
英文摘要	The diversity and activity of sulfate-reducing bacteria (SRB) in the camel gut remains largely unexplored. An abundant SRB community has been previously revealed in the feces of Bactrian camels (Camelus bactrianus). This study aims to combine the 16S rRNA gene profiling, sulfate reduction rate (SRR) measurement with a radioactive tracer, and targeted cultivation to shed light on SRB activity in the camel gut. Fresh feces of 55 domestic Bactrian camels grazing freely on semi-arid mountain pastures in the Kosh-Agach district of the Russian Altai area were analyzed. Feces were sampled in early winter at an ambient temperature of -15 degrees C, which prevented possible contamination. SRR values measured with a radioactive tracer in feces were relatively high and ranged from 0.018 to 0.168 nmol S cm(-3) day(-1). The 16S rRNA gene profiles revealed the presence of Gram-negative Desulfovibrionaceae and spore-forming Desulfotomaculaceae. Targeted isolation allowed us to obtain four pure culture isolates belonging to Desulfovibrio and Desulforamulus. An active SRB community may affect the iron and copper availability in the camel intestine due to metal ions precipitation in the form of sparingly soluble sulfides. The copper-iron sulfide, chalcopyrite (CuFeS2), was detected by X-ray diffraction in 36 out of 55 analyzed camel feces. In semi-arid areas, gypsum, like other evaporite sulfates, can be used as a solid-phase electron acceptor for sulfate reduction in the camel gastrointestinal tract.
英文关键词	sulfate-reduction camels gut microbiota Desulfovibrio Desulforamulus biogenic chalcopyrite
类型	Article
语种	英语
开放获取类型	gold, Green Published
收录类别	SCI-E
WOS记录号	WOS:000940560400001
WOS关键词	HUMAN FECES ; REDUCTION ; DIVERSITY ; SULFIDES ; HEALTHY ; PHASE ; NOV
WOS类目	Microbiology
WOS研究方向	Microbiology
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/397846
推荐引用方式 GB/T 7714	Karnachuk, Olga V.,Panova, Inna A.,Panov, Vasilii L.,et al. Active Sulfate-Reducing Bacterial Community in the Camel Gut[J],2023,11(2).
APA	Karnachuk, Olga V..,Panova, Inna A..,Panov, Vasilii L..,Ikkert, Olga P..,Kadnikov, Vitaly V..,...&Ravin, Nikolai V..(2023).Active Sulfate-Reducing Bacterial Community in the Camel Gut.MICROORGANISMS,11(2).
MLA	Karnachuk, Olga V.,et al."Active Sulfate-Reducing Bacterial Community in the Camel Gut".MICROORGANISMS 11.2(2023).