干旱区生态环境知识资源中心(Arid): Fungi involved in rhinosinusitis in arid regions: insights from molecular identification and antifungal susceptibility

Arid

DOI	10.1128/spectrum.01831-23
	Fungi involved in rhinosinusitis in arid regions: insights from molecular identification and antifungal susceptibility
	Zhou, Shaoqin; Ismail, Mawahib A. I.; Buil, Jochem B.; Gabr, Aida; Verweij, Paul E.; Mahgoub, El-Sheikh; de Hoog, Sybren; Kang, Yingqian; Ahmed, Sarah A.
通讯作者	Zhou, SQ ; Kang, YQ
来源期刊	MICROBIOLOGY SPECTRUM
ISSN	2165-0497
出版年	2023
卷号	11 期号:5
英文摘要	Fungal rhinosinusitis (FRS) is a common problem worldwide, with an increasing burden in arid climate regions. Aspergillus species are the most common causative agents involved. In the present study, we investigated the prevalence, molecular characterization, and antifungal susceptibility of opportunists causing FRS in Sudan on the basis of strains collected over a period of 5 years. beta-Tubulin and calmodulin sequencing were used for species identification, and antifungal susceptibility profiles were evaluated by the protocol of the European Committee on Antimicrobial Susceptibility Testing (EUCAST). Predominant species belonged to the Aspergillus flavus complex (n = 244), A. terreus complex (n = 16), A. fumigatus complex (n = 7), and other fungi (n = 17). Molecular identification of 94 strains of Aspergillus revealed the following species: A. flavus (n = 88), A. terreus (n = 1), A. citrinoterreus (n = 2), A. fumigatus (n = 1), A. caespitosus (n = 1), and A. sydowii (n = 1). Several A. flavus and an A. fumigatus isolates showed reduced susceptibility to azoles (minimum inhibitory concentrations above the clinical breakpoints or epidemiological cutoff values). Despite several mutations revealed in cyp51A of these isolates, none could be directly linked to azole resistance. Molecular identification of fungi causing FRS is useful to identify cryptic species and for epidemiologic studies. IMPORTANCE Fungal rhinosinusitis (FRS) is a significant clinical problem in arid regions. This study provides new insights into the prevalence, etiology, and antifungal susceptibility of FRS pathogens in Sudan, where the disease burden is high. Aspergillus species, particularly the A. flavus complex, were identified as the primary FRS pathogens in the region, with some evidence of antifungal resistance. The molecular identification of fungal species causing FRS is useful for detecting antifungal resistance, identifying cryptic species, and characterizing the epidemiology of the disease. The emergence of Azole resistance Aspergilli in Sudan highlights the need for continued surveillance and appropriate use of antifungal agents. These findings have important implications for clinical management, public health policy, and future research on FRS. Publishing this study in Microbiology Spectrum would enable other researchers and clinicians to build on these findings, ultimately improving the diagnosis, treatment, and prevention of FRS.
英文关键词	Sudan arid climate fungal rhinosinusitis Aspergillus molecular characterization antifungal susceptibility
类型	Article
语种	英语
开放获取类型	Green Published, gold
收录类别	SCI-E
WOS记录号	WOS:001107303900138
WOS关键词	ASPERGILLUS-FUMIGATUS ; EUROPEAN COMMITTEE ; AZOLE RESISTANCE ; ISAVUCONAZOLE
WOS类目	Microbiology
WOS研究方向	Microbiology
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/397838
推荐引用方式 GB/T 7714	Zhou, Shaoqin,Ismail, Mawahib A. I.,Buil, Jochem B.,et al. Fungi involved in rhinosinusitis in arid regions: insights from molecular identification and antifungal susceptibility[J],2023,11(5).
APA	Zhou, Shaoqin.,Ismail, Mawahib A. I..,Buil, Jochem B..,Gabr, Aida.,Verweij, Paul E..,...&Ahmed, Sarah A..(2023).Fungi involved in rhinosinusitis in arid regions: insights from molecular identification and antifungal susceptibility.MICROBIOLOGY SPECTRUM,11(5).
MLA	Zhou, Shaoqin,et al."Fungi involved in rhinosinusitis in arid regions: insights from molecular identification and antifungal susceptibility".MICROBIOLOGY SPECTRUM 11.5(2023).