干旱区生态环境知识资源中心(Arid): Disentangling the Functional Role of Fungi in Cold Seep Sediment

Arid

DOI	10.1128/spectrum.01978-22
	Disentangling the Functional Role of Fungi in Cold Seep Sediment
	Shekarriz, Erfan; Chen, Jiawei; Xu, Zhimeng; Liu, Hongbin
通讯作者	Liu, HB
来源期刊	MICROBIOLOGY SPECTRUM
ISSN	2165-0497
出版年	2023
卷号	11 期号:2
英文摘要	The challenges we face when analyzing eukaryotic metagenomic and metatranscriptomic data sets have hindered our understanding of cold seep fungi and microbial eukaryotes. This fact does not make the mycobiota any less critical in mediating cold seep biogeochemistry. Cold seeps are biological oases of the deep sea fueled by methane, sulfates, nitrates, and other inorganic sources of energy. Chemolithoautotrophic bacteria and archaea dominate seep sediment, and their diversity and biogeochemical functions are well established. Fungi are likewise diverse, metabolically versatile, and known for their ability to capture and oxidize methane. Still, no study has ever explored the functional role of the mycobiota in the cold seep biome. To assess the complex role of fungi and fill in the gaps, we performed network analysis on 147 samples to disentangle fungal-prokaryotic interactions (fungal 18S and prokaryotic 16S) in the Haima cold seep region. We demonstrated that fungi are central species with high connectivity at the epicenter of prokaryotic networks, reduce their random-attack vulnerability by 60%, and enhance information transfer efficiency by 15%. We then scavenged a global metagenomic and metatranscriptomic data set from 10 cold seep regions for fungal genes of interest (hydrophobins, cytochrome P450s, and ligninolytic family of enzymes); this is the first study to report active transcription of 2,500+ fungal genes in the cold seep sediment. The genera Fusarium and Moniliella were of notable importance and directly correlated with high methane abundance in the sulfate-methane transition zone (SMTZ), likely due to their ability to degrade and solubilize methane and oils. Overall, our results highlight the essential yet overlooked contribution of fungi to cold seep biological networks and the role of fungi in regulating cold seep biogeochemistry.IMPORTANCE The challenges we face when analyzing eukaryotic metagenomic and metatranscriptomic data sets have hindered our understanding of cold seep fungi and microbial eukaryotes. This fact does not make the mycobiota any less critical in mediating cold seep biogeochemistry. On the contrary, many fungal genera can oxidize and solubilize methane, produce methane, and play a unique role in nutrient recycling via saprotrophic enzymatic activity. In this study, we used network analysis to uncover key fungal-prokaryotic interactions that can mediate methane biogeochemistry and metagenomics and metatranscriptomics to report that fungi are transcriptionally active in the cold seep sediment. With concerns over rising methane levels and cold seeps being a pivotal source of global methane input, our holistic understanding of methane biogeochemistry with all domains of life is essential. We ultimately encourage scientists to utilize state-of-the-art tools and multifaceted approaches to uncover the role of microeukaryotic organisms in understudied systems.
英文关键词	biogeochemistry cold seep filamentous fungi fungi metagenomics metatranscriptomics methanotrophs network analysis nonhuman microbiome target amplicon sequencing
类型	Article
语种	英语
开放获取类型	Green Published, gold
收录类别	SCI-E
WOS记录号	WOS:000947772500001
WOS关键词	MOLECULAR EVIDENCE ; SEA ; EVOLUTION ; REPRODUCTION ; DIVERSITY
WOS类目	Microbiology
WOS研究方向	Microbiology
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/397837
推荐引用方式 GB/T 7714	Shekarriz, Erfan,Chen, Jiawei,Xu, Zhimeng,et al. Disentangling the Functional Role of Fungi in Cold Seep Sediment[J],2023,11(2).
APA	Shekarriz, Erfan,Chen, Jiawei,Xu, Zhimeng,&Liu, Hongbin.(2023).Disentangling the Functional Role of Fungi in Cold Seep Sediment.MICROBIOLOGY SPECTRUM,11(2).
MLA	Shekarriz, Erfan,et al."Disentangling the Functional Role of Fungi in Cold Seep Sediment".MICROBIOLOGY SPECTRUM 11.2(2023).