干旱区生态环境知识资源中心(Arid): High-Throughput Absolute Quantification Sequencing Reveals that a Combination of Leguminous Shrubs Is Effective in Driving Soil Bacterial Diversity During the Process of Desertification Reversal

Arid

DOI	10.1007/s00248-022-02151-0
	High-Throughput Absolute Quantification Sequencing Reveals that a Combination of Leguminous Shrubs Is Effective in Driving Soil Bacterial Diversity During the Process of Desertification Reversal
	Liu, Wangsuo; Qiu, Kaiyang; Xie, Yingzhong; Huang, Yeyun; Wang, Ruixia; Li, Haichao; Meng, Wenfen; He, Yi; Li, Yayuan; Li, Haiquan; Zhao, Pengbo; Yang, Yi
通讯作者	Qiu, KY
来源期刊	MICROBIAL ECOLOGY
ISSN	0095-3628
EISSN	1432-184X
出版年	2023
卷号	86 期号:2 页码:1145-1163
英文摘要	Desertification leads to the extreme fragility of ecosystems and seriously threatens ecosystem functioning in desert areas. The planting of xerophytes, especially leguminous shrubs, is an effective and common means to reverse desertification. Soil microorganisms play a crucial role in nutrient cycling and energy flow in ecosystems. However, the effects of introducing leguminous shrubs on soil microbial diversity and the relevant mechanisms are not clear. Here, we employed the high-throughput absolute quantification 16S rRNA sequencing method to analyze the diversity of soil bacteria in sand-fixing areas of mixed shrublands with three combinations of shrubs, i.e., C. korshinskii x Corethrodendron scoparium (CaKCoS), C. korshinskii x Calligonum mongolicum (CaKCaM), and C. scoparium x C. mongolicum (CoSCaM), in the south of the Mu Us Sandy Land, China. This area suffered from moving dunes 20 years ago, but after introducing these shrubs to fix the dunes, the ecosystem was restored. Additionally, the effects of soil physicochemical properties on soil bacterial composition and diversity were analyzed with redundancy analysis (RDA) and structural equation modeling (SEM). It was found that the Shannon index of soil bacteria in CaKCoS was significantly higher than that in CaKCaM and CoSCaM, and the abundance of the dominant phyla, including Actinobacteria, Proteobacteria, Acidobacteria, Chloroflexi, Planctomycetes, Thaumarchaeota, Armatimonadetes, candidate_division_WPS-1, and Nitrospirae, increased significantly in CaKCoS and CaKCaM compared to that in CoSCaM. RDA showed that the majority of soil properties, such as total nitrogen (TN), available potassium (AK), N:P ratio, soil moisture (SM), and available phosphorus (AP), were important soil environmental factors affecting the abundance of the dominant phyla, and RDA1 and RDA2 accounted for 56.66% and 2.35% of the total variation, respectively. SEM showed that the soil bacterial alpha-diversity was positively affected by the soil organic carbon (SOC), N:P ratio, and total phosphorus (TP). Moreover, CaKCoS had higher SM, total carbon (TC), total potassium (TK), and AP than CaKCaM and CoSCaM. Collectively, these results highlight a conceptual framework in which the combination of leguminous shrubs can effectively drive soil bacterial diversity by improving soil physicochemical properties and maintaining ecosystem functioning during desertification reversal.
英文关键词	Combination of leguminous shrubs Soil bacterial diversity Absolute quantification sequencing Edaphic factor Sand-fixing afforestation Straw checkerboard
类型	Article
语种	英语
开放获取类型	hybrid, Green Published
收录类别	SCI-E
WOS记录号	WOS:000897447900001
WOS关键词	RESOURCE ISLANDS ; SAND DUNE ; COMBATING DESERTIFICATION ; MICROBIAL COMMUNITIES ; CNP STOICHIOMETRY ; PLANT TRAITS ; DESERT ; ABUNDANCE ; RESPONSES ; LAND
WOS类目	Ecology ; Marine & Freshwater Biology ; Microbiology
WOS研究方向	Environmental Sciences & Ecology ; Marine & Freshwater Biology ; Microbiology
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/397836
推荐引用方式 GB/T 7714	Liu, Wangsuo,Qiu, Kaiyang,Xie, Yingzhong,et al. High-Throughput Absolute Quantification Sequencing Reveals that a Combination of Leguminous Shrubs Is Effective in Driving Soil Bacterial Diversity During the Process of Desertification Reversal[J],2023,86(2):1145-1163.
APA	Liu, Wangsuo.,Qiu, Kaiyang.,Xie, Yingzhong.,Huang, Yeyun.,Wang, Ruixia.,...&Yang, Yi.(2023).High-Throughput Absolute Quantification Sequencing Reveals that a Combination of Leguminous Shrubs Is Effective in Driving Soil Bacterial Diversity During the Process of Desertification Reversal.MICROBIAL ECOLOGY,86(2),1145-1163.
MLA	Liu, Wangsuo,et al."High-Throughput Absolute Quantification Sequencing Reveals that a Combination of Leguminous Shrubs Is Effective in Driving Soil Bacterial Diversity During the Process of Desertification Reversal".MICROBIAL ECOLOGY 86.2(2023):1145-1163.