干旱区生态环境知识资源中心(Arid): Nanohaloarchaea as beneficiaries of xylan degradation by haloarchaea

Arid

DOI	10.1111/1751-7915.14272
	Nanohaloarchaea as beneficiaries of xylan degradation by haloarchaea
	La Cono, Violetta; Messina, Enzo; Reva, Oleg; Smedile, Francesco; La Spada, Gina; Crisafi, Francesca; Marturano, Laura; Miguez, Noa; Ferrer, Manuel; Selivanova, Elena A. A.; Golyshina, Olga V. V.; Golyshin, Peter N. N.; Rohde, Manfred; Krupovic, Mart; Merkel, Alexander Y. Y.; Sorokin, Dimitry Y. Y.; Hallsworth, John E. E.; Yakimov, Michail M. M.
通讯作者	Yakimov, MM
来源期刊	MICROBIAL BIOTECHNOLOGY
ISSN	1751-7915
出版年	2023
卷号	16 期号:9 页码:1803-1822
英文摘要	Climate change, desertification, salinisation of soils and the changing hydrology of the Earth are creating or modifying microbial habitats at all scales including the oceans, saline groundwaters and brine lakes. In environments that are saline or hypersaline, the biodegradation of recalcitrant plant and animal polysaccharides can be inhibited by salt-induced microbial stress and/or by limitation of the metabolic capabilities of halophilic microbes. We recently demonstrated that the chitinolytic haloarchaeon Halomicrobium can serve as the host for an ectosymbiont, nanohaloarchaeon 'Candidatus Nanohalobium constans'. Here, we consider whether nanohaloarchaea can benefit from the haloarchaea-mediated degradation of xylan, a major hemicellulose component of wood. Using samples of natural evaporitic brines and anthropogenic solar salterns, we describe genome-inferred trophic relations in two extremely halophilic xylan-degrading three-member consortia. We succeeded in genome assembly and closure for all members of both xylan-degrading cultures and elucidated the respective food chains within these consortia. We provide evidence that ectosymbiontic nanohaloarchaea is an active ecophysiological component of extremely halophilic xylan-degrading communities (although by proxy) in hypersaline environments. In each consortium, nanohaloarchaea occur as ectosymbionts of Haloferax, which in turn act as scavenger of oligosaccharides produced by xylan-hydrolysing Halorhabdus. We further obtained and characterised the nanohaloarchaea-host associations using microscopy, multi-omics and cultivation approaches. The current study also doubled culturable nanohaloarchaeal symbionts and demonstrated that these enigmatic nano-sized archaea can be readily isolated in binary co-cultures using an appropriate enrichment strategy. We discuss the implications of xylan degradation by halophiles in biotechnology and for the United Nation's Sustainable Development Goals.
类型	Article
语种	英语
开放获取类型	Green Published, gold
收录类别	SCI-E
WOS记录号	WOS:001010155900001
WOS关键词	ARCHAEA ; LIMITS ; CLASSIFICATION ; COMMUNITIES ; DIVERSITY ; CELLULASE ; BACTERIA ; ACCURATE ; SILVA ; LIFE
WOS类目	Biotechnology & Applied Microbiology ; Microbiology
WOS研究方向	Biotechnology & Applied Microbiology ; Microbiology
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/397833
推荐引用方式 GB/T 7714	La Cono, Violetta,Messina, Enzo,Reva, Oleg,et al. Nanohaloarchaea as beneficiaries of xylan degradation by haloarchaea[J],2023,16(9):1803-1822.
APA	La Cono, Violetta.,Messina, Enzo.,Reva, Oleg.,Smedile, Francesco.,La Spada, Gina.,...&Yakimov, Michail M. M..(2023).Nanohaloarchaea as beneficiaries of xylan degradation by haloarchaea.MICROBIAL BIOTECHNOLOGY,16(9),1803-1822.
MLA	La Cono, Violetta,et al."Nanohaloarchaea as beneficiaries of xylan degradation by haloarchaea".MICROBIAL BIOTECHNOLOGY 16.9(2023):1803-1822.