干旱区生态环境知识资源中心(Arid): Plasmodium falciparum population structure inferred by msp1 amplicon sequencing of parasites collected from febrile patients in Kenya

Arid

DOI	10.1186/s12936-023-04700-5
	Plasmodium falciparum population structure inferred by msp1 amplicon sequencing of parasites collected from febrile patients in Kenya
	Andika, Brian; Mobegi, Victor; Gathii, Kimita; Nyataya, Josphat; Maina, Naomi; Awinda, George; Mutai, Beth; Waitumbi, John
通讯作者	Waitumbi, J
来源期刊	MALARIA JOURNAL
EISSN	1475-2875
出版年	2023
卷号	22 期号:1
英文摘要	Background Multiplicity of infection (MOI) is an important measure of Plasmodium falciparum diversity, usually derived from the highly polymorphic genes, such as msp1, msp2 and glurp as well as microsatellites. Conventional methods of deriving MOI lack fine resolution needed to discriminate minor clones. This study used amplicon sequencing (AmpliSeq) of P. falciparum msp1 (Pfmsp1) to measure spatial and temporal genetic diversity of P. falciparum. Methods 264 P. falciparum positive blood samples collected from areas of differing malaria endemicities between 2010 and 2019 were used. Pfmsp1 gene was amplified and amplicon libraries sequenced on Illumina MiSeq. Sequences were aligned against a reference sequence (NC_004330.2) and clustered to detect fragment length polymorphism and amino acid variations. Results Children < 5 years had higher parasitaemia (median = 23.5 +/- 5 SD, p = 0.03) than the > 5-14 (= 25.3 +/- 5 SD), and those > 15 (= 25.1 +/- 6 SD). Of the alleles detected, 553 (54.5%) were K1, 250 (24.7%) MAD20 and 211 (20.8%) RO33 that grouped into 19 K1 allelic families (108-270 bp), 14 MAD20 (108-216 bp) and one RO33 (153 bp). AmpliSeq revealed nucleotide polymorphisms in alleles that had similar sizes, thus increasing the K1 to 104, 58 for MAD20 and 14 for RO33. By AmpliSeq, the mean MOI was 4.8 (+/- 0.78, 95% CI) for the malaria endemic Lake Victoria region, 4.4 (+/- 1.03, 95% CI) for the epidemic prone Kisii Highland and 3.4 (+/- 0.62, 95% CI) for the seasonal malaria Semi-Arid region. MOI decreased with age: 4.5 (+/- 0.76, 95% CI) for children < 5 years, compared to 3.9 (+/- 0.70, 95% CI) for ages 5 to 14 and 2.7 (+/- 0.90, 95% CI) for those > 15. Females' MOI (4.2 +/- 0.66, 95% CI) was not different from males 4.0 (+/- 0.61, 95% CI). In all regions, the number of alleles were high in the 2014-2015 period, more so in the Lake Victoria and the seasonal transmission arid regions. Conclusion These findings highlight the added advantages of AmpliSeq in haplotype discrimination and the associated improvement in unravelling complexity of P. falciparum population structure.
英文关键词	Malaria Multiplicity of infection P. falciparum P. falciparummsp1 Deep sequencing Genetic diversity
类型	Article
语种	英语
开放获取类型	gold, Green Published, Green Submitted
收录类别	SCI-E
WOS记录号	WOS:001066612600002
WOS关键词	MEROZOITE SURFACE PROTEIN-1 ; GENETIC DIVERSITY ; MALARIA ; ANTIGEN ; INFECTIONS ; GENOTYPES ; MILD ; AREA
WOS类目	Infectious Diseases ; Parasitology ; Tropical Medicine
WOS研究方向	Infectious Diseases ; Parasitology ; Tropical Medicine
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/397800
推荐引用方式 GB/T 7714	Andika, Brian,Mobegi, Victor,Gathii, Kimita,et al. Plasmodium falciparum population structure inferred by msp1 amplicon sequencing of parasites collected from febrile patients in Kenya[J],2023,22(1).
APA	Andika, Brian.,Mobegi, Victor.,Gathii, Kimita.,Nyataya, Josphat.,Maina, Naomi.,...&Waitumbi, John.(2023).Plasmodium falciparum population structure inferred by msp1 amplicon sequencing of parasites collected from febrile patients in Kenya.MALARIA JOURNAL,22(1).
MLA	Andika, Brian,et al."Plasmodium falciparum population structure inferred by msp1 amplicon sequencing of parasites collected from febrile patients in Kenya".MALARIA JOURNAL 22.1(2023).