干旱区生态环境知识资源中心(Arid): Transcriptome Analysis Reveals Key Molecular Pathways in Response to Alkaline Salt Stress in Canola (Brassica napus L.) Roots

Arid

DOI	10.1007/s00344-022-10774-3
	Transcriptome Analysis Reveals Key Molecular Pathways in Response to Alkaline Salt Stress in Canola (Brassica napus L.) Roots
	Wang, Weichao; Pang, Jiayin; Zhang, Fenghua; Sun, Lupeng; Yang, Lei; Fu, Tingdong; Siddique, Kadambot H. M.
通讯作者	Zhang, FH
来源期刊	JOURNAL OF PLANT GROWTH REGULATION
ISSN	0721-7595
EISSN	1435-8107
出版年	2023
卷号	42 期号:5 页码:3111-3127
英文摘要	Soil salinity is a major constraint to crop growth and productivity, limiting sustainable agriculture in arid regions. Understanding the molecular mechanisms underlying salinity tolerance in canola is important for improving salt tolerance and promoting canola cultivation in saline soils. This study exposed canola seedlings to 40 mM Na2CO3 for varying durations before collecting the roots for RNA-Seq analysis and qRT-PCR validation. The results showed that transcript expression in canola roots differed at different stages of Na2CO3 exposure, with oxidative stress responses and sugar metabolism (energy supply) evident after 2 h and increased amino acid metabolism and organic acid metabolism evident after 24 and 72 h. The Na2CO3 treatments increased the expression of numerous differential genes that enrich Ca2+, abscisic acid (ABA), and reactive oxygen species (ROS) signaling pathways. In addition, several transcription factor families associated with Na2CO3 tolerance were identified, including bHLH, WRKY, ERF, MYB, and NAC. In summary, crosstalk between Ca2+ signaling pathways and ABA and ROS signaling pathways induced the expression of downstream genes and produced osmoregulatory substances (organic acids) that further regulate canola tolerance to alkaline salt stress. These results provide a basis for further studies on the regulatory mechanisms of alkaline salt stress adaptation in canola.
英文关键词	Alkaline stress Transcriptomics Ca2+ signaling Transcription factors Organic acids
类型	Article
语种	英语
收录类别	SCI-E
WOS记录号	WOS:000849469000002
WOS关键词	OXIDATIVE STRESS ; ION ACCUMULATION ; TOLERANCE ; SALINITY ; DROUGHT ; ARABIDOPSIS ; GERMINATION ; GROWTH ; ANTIOXIDANTS ; STRATEGIES
WOS类目	Plant Sciences
WOS研究方向	Plant Sciences
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/397509
推荐引用方式 GB/T 7714	Wang, Weichao,Pang, Jiayin,Zhang, Fenghua,et al. Transcriptome Analysis Reveals Key Molecular Pathways in Response to Alkaline Salt Stress in Canola (Brassica napus L.) Roots[J],2023,42(5):3111-3127.
APA	Wang, Weichao.,Pang, Jiayin.,Zhang, Fenghua.,Sun, Lupeng.,Yang, Lei.,...&Siddique, Kadambot H. M..(2023).Transcriptome Analysis Reveals Key Molecular Pathways in Response to Alkaline Salt Stress in Canola (Brassica napus L.) Roots.JOURNAL OF PLANT GROWTH REGULATION,42(5),3111-3127.
MLA	Wang, Weichao,et al."Transcriptome Analysis Reveals Key Molecular Pathways in Response to Alkaline Salt Stress in Canola (Brassica napus L.) Roots".JOURNAL OF PLANT GROWTH REGULATION 42.5(2023):3111-3127.