干旱区生态环境知识资源中心(Arid): Xerophytic Lichens from Gypsiferous Outcrops of Arid Areas of Andalusia as a Source of Anti-Phytopathogenic Depsides

Arid

DOI	10.3390/jof9090887
	Xerophytic Lichens from Gypsiferous Outcrops of Arid Areas of Andalusia as a Source of Anti-Phytopathogenic Depsides
	Fernandez-Pastor, Ignacio; Gonzalez-Menendez, Victor; Martinez Andrade, Kevin; Serrano, Rachel; Mackenzie, Thomas A.; Benitez, Guillermo; Casares-Porcel, Manuel; Genilloud, Olga; Reyes, Fernando
通讯作者	González-Menéndez, V ; Reyes, F
来源期刊	JOURNAL OF FUNGI
EISSN	2309-608X
出版年	2023
卷号	9 期号:9
英文摘要	In a survey to evaluate the potential of lichens associated with gypsum areas as sources of new antifungal metabolites, six species of lichens were collected in the gypsum outcrops of the Sorbas Desert (Diploschistes ocellatus and Seirophora lacunosa) and the Tabernas Desert (Cladonia foliacea, Acarospora placodiformis, Squamarina lentigera and Xanthoparmelia pokornyi) in southern Spain. Raw lichen acetone extracts were tested against a panel of seven phytopathogenic fungi, including Botrytis cinerea, Colletotrichum acutatum, Fusarium oxysporum f.sp cubense TR4, Fusarium ploriferaum, Magnaporthe grisea, Verticillium dahliae and Zymoseptoria tritici. Active extracts of Cladonia foliacea, Xanthoparmelia pokornyi and Squamarina lentigera were analyzed by HPLC-MS/MS and Molecular Networking to identify possible metabolites responsible for the antifungal activity. A total of ten depside-like metabolites were identified by MS/MS dereplication and NMR experiments, of which one was a new derivative of fumaroprotocetraric acid. The compounds without previously described biological activity were purified and tested against the panel of fungal phytopathogens. Herein, the antifungal activity against fungal phytopathogens of 4 '-O-methylpaludosic acid, divaricatic acid and stenosporic acid is reported for the first time. Stenosporic and divaricatic acids displayed a broad antifungal spectrum against seven relevant fungal phytopathogens in a micromolar range, including the extremely resistant fungus F. oxysporum f. sp. cubense Tropical Race 4 (TR4). 4 '-O-methylpaludosic acid exhibited specific antifungal activity against the wheat pathogen Z. tritici, with an IC50 of 38.87 mu g/mL (87.1 mu M) in the absorbance-based assay and 24.88 mu g/mL (55.52 mu M) in the fluorescence-based assay.
英文关键词	xerophytic lichens antifungal activity biopesticides fungal phytopathogens dereplication MS/MS molecular networking depsides
类型	Article
语种	英语
开放获取类型	gold, Green Published
收录类别	SCI-E
WOS记录号	WOS:001081043200001
WOS关键词	ANTIMICROBIAL ACTIVITY ; ANTIOXIDANT ACTIVITY ; STENOSPORIC ACID ; EXTRACTS ; METABOLITES
WOS类目	Microbiology ; Mycology
WOS研究方向	Microbiology ; Mycology
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/397333
推荐引用方式 GB/T 7714	Fernandez-Pastor, Ignacio,Gonzalez-Menendez, Victor,Martinez Andrade, Kevin,et al. Xerophytic Lichens from Gypsiferous Outcrops of Arid Areas of Andalusia as a Source of Anti-Phytopathogenic Depsides[J],2023,9(9).
APA	Fernandez-Pastor, Ignacio.,Gonzalez-Menendez, Victor.,Martinez Andrade, Kevin.,Serrano, Rachel.,Mackenzie, Thomas A..,...&Reyes, Fernando.(2023).Xerophytic Lichens from Gypsiferous Outcrops of Arid Areas of Andalusia as a Source of Anti-Phytopathogenic Depsides.JOURNAL OF FUNGI,9(9).
MLA	Fernandez-Pastor, Ignacio,et al."Xerophytic Lichens from Gypsiferous Outcrops of Arid Areas of Andalusia as a Source of Anti-Phytopathogenic Depsides".JOURNAL OF FUNGI 9.9(2023).