干旱区生态环境知识资源中心(Arid): Volatile Metabolites in Lavage Fluid Are Correlated with Cytokine Production in a Valley Fever Murine Model

Arid

DOI	10.3390/jof9010115
	Volatile Metabolites in Lavage Fluid Are Correlated with Cytokine Production in a Valley Fever Murine Model
	Keppler, Emily A. Higgins A.; Van Dyke, Marley C. Caballero; Mead, Heather L.; Lake, Douglas F.; Magee, D. Mitchell; Barker, Bridget M.; Bean, Heather D.
通讯作者	Bean, HD
来源期刊	JOURNAL OF FUNGI
EISSN	2309-608X
出版年	2023
卷号	9 期号:1
英文摘要	Coccidioides immitis and Coccidioides posadasii are soil-dwelling fungi of arid regions in North and South America that are responsible for Valley fever (coccidioidomycosis). Forty percent of patients with Valley fever exhibit symptoms ranging from mild, self-limiting respiratory infections to severe, life-threatening pneumonia that requires treatment. Misdiagnosis as bacterial pneumonia commonly occurs in symptomatic Valley fever cases, resulting in inappropriate treatment with antibiotics, increased medical costs, and delay in diagnosis. In this proof-of-concept study, we explored the feasibility of developing breath-based diagnostics for Valley fever using a murine lung infection model. To investigate potential volatile biomarkers of Valley fever that arise from host-pathogen interactions, we infected C57BL/6J mice with C. immitis RS (n = 6), C. posadasii Silveira (n = 6), or phosphate-buffered saline (n = 4) via intranasal inoculation. We measured fungal dissemination and collected bronchoalveolar lavage fluid (BALF) for cytokine profiling and for untargeted volatile metabolomics via solid-phase microextraction (SPME) and two-dimensional gas chromatography coupled with time-of-flight mass spectrometry (GCxGC-TOFMS). We identified 36 volatile organic compounds (VOCs) that were significantly correlated (p < 0.05) with cytokine abundance. These 36 VOCs clustered mice by their cytokine production and were also able to separate mice with moderate-to-high cytokine production by infection strain. The data presented here show that Coccidioides and/or the host produce volatile metabolites that may yield biomarkers for a Valley fever breath test that can detect coccidioidal infection and provide clinically relevant information on primary pulmonary disease severity.
英文关键词	Valley fever coccidioidomycosis fungal pneumonia mouse model biomarkers comprehensive two-dimensional gas chromatography GCxGC untargeted metabolomics
类型	Article
语种	英语
开放获取类型	gold, Green Published
收录类别	SCI-E
WOS记录号	WOS:000914676100001
WOS关键词	ORGANIC-COMPOUNDS MVOCS ; GAS-CHROMATOGRAPHY ; IMMUNE-RESPONSES ; INNATE RESPONSES ; IDENTIFICATION ; COCCIDIOIDOMYCOSIS ; DIAGNOSIS ; GROWTH ; MICE ; RESISTANCE
WOS类目	Microbiology ; Mycology
WOS研究方向	Microbiology ; Mycology
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/397329
推荐引用方式 GB/T 7714	Keppler, Emily A. Higgins A.,Van Dyke, Marley C. Caballero,Mead, Heather L.,et al. Volatile Metabolites in Lavage Fluid Are Correlated with Cytokine Production in a Valley Fever Murine Model[J],2023,9(1).
APA	Keppler, Emily A. Higgins A..,Van Dyke, Marley C. Caballero.,Mead, Heather L..,Lake, Douglas F..,Magee, D. Mitchell.,...&Bean, Heather D..(2023).Volatile Metabolites in Lavage Fluid Are Correlated with Cytokine Production in a Valley Fever Murine Model.JOURNAL OF FUNGI,9(1).
MLA	Keppler, Emily A. Higgins A.,et al."Volatile Metabolites in Lavage Fluid Are Correlated with Cytokine Production in a Valley Fever Murine Model".JOURNAL OF FUNGI 9.1(2023).