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DOI	10.1111/1556-4029.15279
	Rapid enantiomeric analysis of zopiclone in serum by supercritical fluid chromatography-tandem mass spectrometry
	Segawa, Hiroki; Iwata, Yuko T.; Okada, Yuki; Yamamuro, Tadashi; Kuwayama, Kenji; Tsujikawa, Kenji; Kanamori, Tatsuyuki
通讯作者	Segawa, H
来源期刊	JOURNAL OF FORENSIC SCIENCES
ISSN	0022-1198
EISSN	1556-4029
出版年	2023
卷号	68 期号:4 页码:1352-1358
英文摘要	Zopiclone (ZOP) is a hypnotic drug prescribed to treat insomnia. Due to the chiral nature of ZOP, the psychologically active S-form and inactive R-form need to be determined enantiomerically in a forensic drug analysis. In the present study, a supercritical fluid chromatography (SFC) method was designed with a faster analysis ability than that of previously reported techniques. The SFC-tandem mass spectrometry (SFC-MS/MS) method was optimized using a column with a chiral polysaccharide stationary phase (Trefoil CEL2). ZOP was extracted from pooled human serum using solid-phase extraction (Oasis HLB) and analyzed. The developed SFC-MS/MS method achieved the baseline separation of S-ZOP and R-ZOP within 2 min. The fit-for-purpose method validation indicated that the optimized solid-phase extraction achieved near complete recovery and approximately 70% of the matrix effect. Both the retention time and peak area showed sufficient precision. The lower and upper limits of quantification (LOQ) were 5.7 x 10(-2) ng/mL and 25 ng/mL for R-ZOP, and 5.2 x 10(-2) ng/mL and 25 ng/mL for S-ZOP. The calibration line was linear in the range from lower LOQ to upper LOQ. The stability test indicated that ZOP in serum stored in a refrigerator (4 degrees C) degraded and about 55% remained in 31 days. The quick analysis of the SFC-MS/MS method makes it a valid option for the enantiomeric analysis of ZOP.
英文关键词	chiral analysis mass spectrometry MS SFC supercritical fluid chromatography zopiclone
类型	Article
语种	英语
收录类别	SCI-E
WOS记录号	WOS:000989806600001
WOS关键词	QUANTITATIVE ANALYTICAL PROCEDURES ; SFSTP PROPOSAL ; ENANTIOSELECTIVE DETERMINATION ; N-OXIDE ; METABOLITES ; VALIDATION ; URINE ; HARMONIZATION ; DEGRADATION ; STRATEGIES
WOS类目	Medicine, Legal
WOS研究方向	Legal Medicine
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/397325
推荐引用方式 GB/T 7714	Segawa, Hiroki,Iwata, Yuko T.,Okada, Yuki,et al. Rapid enantiomeric analysis of zopiclone in serum by supercritical fluid chromatography-tandem mass spectrometry[J],2023,68(4):1352-1358.
APA	Segawa, Hiroki.,Iwata, Yuko T..,Okada, Yuki.,Yamamuro, Tadashi.,Kuwayama, Kenji.,...&Kanamori, Tatsuyuki.(2023).Rapid enantiomeric analysis of zopiclone in serum by supercritical fluid chromatography-tandem mass spectrometry.JOURNAL OF FORENSIC SCIENCES,68(4),1352-1358.
MLA	Segawa, Hiroki,et al."Rapid enantiomeric analysis of zopiclone in serum by supercritical fluid chromatography-tandem mass spectrometry".JOURNAL OF FORENSIC SCIENCES 68.4(2023):1352-1358.