干旱区生态环境知识资源中心(Arid): Transcriptomic and metabolomic analysis reveals that symbiotic nitrogen fixation enhances drought resistance in common bean

Arid

DOI	10.1093/jxb/erad083
	Transcriptomic and metabolomic analysis reveals that symbiotic nitrogen fixation enhances drought resistance in common bean
	Lopez, Cristina Ma; Alseekh, Saleh; Torralbo, Fernando; Rivas, Felix J. Martinez; Fernie, Alisdair R.; Amil-Ruiz, Francisco; Alamillo, Josefa M.
通讯作者	Alamillo, JM
来源期刊	JOURNAL OF EXPERIMENTAL BOTANY
ISSN	0022-0957
EISSN	1460-2431
出版年	2023
卷号	74 期号:10 页码:3203-3219
英文摘要	Common bean (Phaseolus vulgaris L.), one of the most important legume crops, uses atmospheric nitrogen through symbiosis with soil rhizobia, reducing the need for nitrogen fertilization. However, this legume is particularly sensitive to drought conditions, prevalent in arid regions where this crop is cultured. Therefore, studying the response to drought is important to sustain crop productivity. We have used integrated transcriptomic and metabolomic analysis to understand the molecular responses to water deficit in a marker-class common bean accession cultivated under N-2 fixation or fertilized with nitrate (NO3-). RNA-seq revealed more transcriptional changes in the plants fertilized with NO3- than in the N-2-fixing plants. However, changes in N-2-fixing plants were more associated with drought tolerance than in those fertilized with NO3-. N-2-fixing plants accumulated more ureides in response to drought, and GC/MS and LC/MS analysis of primary and secondary metabolite profiles revealed that N-2-fixing plants also had higher levels of abscisic acid, proline, raffinose, amino acids, sphingolipids, and triacylglycerols than those fertilized with NO3-. Moreover, plants grown under nitrogen fixation recovered from drought better than plants fertilized with NO3-. Altogether we show that common bean plants grown under symbiotic nitrogen fixation were more protected against drought than the plants fertilized with nitrate. Integrated transcriptomic and metabolomic analysis shows that common beans grown under symbiotic nitrogen fixation respond more efficiently against drought stress than plants fertilized with NO3-.
英文关键词	Drought tolerance metabolomics N-2 fixation nitrate Phaseolus vulgaris transcriptomic ureides
类型	Article
语种	英语
收录类别	SCI-E
WOS记录号	WOS:000956302200001
WOS关键词	ABSCISIC-ACID BIOSYNTHESIS ; LEAF SENESCENCE ; ABIOTIC STRESS ; UREIDE ACCUMULATION ; PURINE METABOLITE ; GENE-EXPRESSION ; N-2 FIXATION ; ARABIDOPSIS ; INHIBITION ; TOLERANCE
WOS类目	Plant Sciences
WOS研究方向	Plant Sciences
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/397318
推荐引用方式 GB/T 7714	Lopez, Cristina Ma,Alseekh, Saleh,Torralbo, Fernando,et al. Transcriptomic and metabolomic analysis reveals that symbiotic nitrogen fixation enhances drought resistance in common bean[J],2023,74(10):3203-3219.
APA	Lopez, Cristina Ma.,Alseekh, Saleh.,Torralbo, Fernando.,Rivas, Felix J. Martinez.,Fernie, Alisdair R..,...&Alamillo, Josefa M..(2023).Transcriptomic and metabolomic analysis reveals that symbiotic nitrogen fixation enhances drought resistance in common bean.JOURNAL OF EXPERIMENTAL BOTANY,74(10),3203-3219.
MLA	Lopez, Cristina Ma,et al."Transcriptomic and metabolomic analysis reveals that symbiotic nitrogen fixation enhances drought resistance in common bean".JOURNAL OF EXPERIMENTAL BOTANY 74.10(2023):3203-3219.