干旱区生态环境知识资源中心(Arid): The Transcriptomic Analysis of the Response of Pinus massoniana to Drought Stress and a Functional Study on the ERF1 Transcription Factor

Arid

DOI	10.3390/ijms241311103
	The Transcriptomic Analysis of the Response of Pinus massoniana to Drought Stress and a Functional Study on the ERF1 Transcription Factor
	Zhang, Jinfeng; Wang, Dengbao; Chen, Peizhen; Zhang, Chi; Yao, Sheng; Hao, Qingqing; Agassin, Romaric Hippolyte; Ji, Kongshu
通讯作者	Ji, KS
来源期刊	INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
ISSN	1661-6596
EISSN	1422-0067
出版年	2023
卷号	24 期号:13
英文摘要	Pinus massoniana is a major fast-growing timber tree species planted in arid areas of south China, which has a certain drought-resistant ability. However, severe drought and long-term water shortage limit its normal growth and development. Therefore, in this study, physiological indices, and the transcriptome sequencing and cloning of AP2/ERF transcription factor of P. massonsiana were determined to clarify its molecular mechanism of drought stress. The results showed that stomatal conductance (Gs) content was significantly decreased, and superoxide dismutase (SOD) activity, and malondialdehyde (MDA) and abscisic acid (ABA) content were significantly increased under drought stress. Transcriptomic analysis revealed that compared to the control, 9, 3550, and 4142 unigenes with differential expression were identified by comparing plants subjected to light, moderate or severe drought. AP2/ERF with high expression was screened out for cloning. To investigate the biological functions of ERF1, it was over-expressed in wild-type Populus davdianaand x P. bolleana via the leaf disc method. Under drought stress, compared to wild-type plants, ERF1 over-expressing poplar lines (OE) maintained a higher photosynthetic rate and growth, while the transpiration rate and stomatal conductance significantly decreased and water use efficiency was improved, indicating that drought tolerance was enhanced. This study provides an insight into the molecular mechanism of drought stress adaptation in P. massoniana.
英文关键词	Pinus massoniana Lamb drought stress transcriptome sequencing transcription factor AP2 ERF
类型	Article
语种	英语
开放获取类型	Green Published, gold
收录类别	SCI-E
WOS记录号	WOS:001028098800001
WOS关键词	WATER-USE EFFICIENCY ; TOLERANCE ; EXPRESSION ; BINDING ; PLANTS
WOS类目	Biochemistry & Molecular Biology ; Chemistry, Multidisciplinary
WOS研究方向	Biochemistry & Molecular Biology ; Chemistry
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/397019
推荐引用方式 GB/T 7714	Zhang, Jinfeng,Wang, Dengbao,Chen, Peizhen,et al. The Transcriptomic Analysis of the Response of Pinus massoniana to Drought Stress and a Functional Study on the ERF1 Transcription Factor[J],2023,24(13).
APA	Zhang, Jinfeng.,Wang, Dengbao.,Chen, Peizhen.,Zhang, Chi.,Yao, Sheng.,...&Ji, Kongshu.(2023).The Transcriptomic Analysis of the Response of Pinus massoniana to Drought Stress and a Functional Study on the ERF1 Transcription Factor.INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,24(13).
MLA	Zhang, Jinfeng,et al."The Transcriptomic Analysis of the Response of Pinus massoniana to Drought Stress and a Functional Study on the ERF1 Transcription Factor".INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 24.13(2023).