干旱区生态环境知识资源中心(Arid): Neutral and Pectic Heteropolysaccharides Isolated from Opuntia joconostle Mucilage: Composition, Molecular Dimensions and Prebiotic Potential

Arid

DOI	10.3390/ijms24043208
	Neutral and Pectic Heteropolysaccharides Isolated from Opuntia joconostle Mucilage: Composition, Molecular Dimensions and Prebiotic Potential
	Cruz-Rubio, Jose Manuel; Riva, Alessandra; Cybulska, Justyna; Zdunek, Artur; Berry, David; Loeppert, Renate; Viernstein, Helmut; Praznik, Werner; Maghuly, Fatemeh
通讯作者	Cruz-Rubio, JM ; Maghuly, F
来源期刊	INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
EISSN	1422-0067
出版年	2023
卷号	24 期号:4
英文摘要	Opuntia joconostle is a semi-wild cactus cultivated for its fruit. However, the cladodes are often discarded, wasting the potentially useful mucilage in them. The mucilage is composed primarily of heteropolysaccharides, characterized by their molar mass distribution, monosaccharide composition, structural features (by vibrational spectroscopy, FT IR, and atomic force microscopy, AFM), and fermentability by known saccharolytic commensal members of the gut microbiota. After fractionation with ion exchange chromatography, four polysaccharides were found: one neutral (composed mainly of galactose, arabinose, and xylose) and three acidic, with a galacturonic acid content from 10 to 35%(mol). Their average molar masses ranged from 1.8 x 10(5) to 2.8 x 10(5) g center dot mol(-1). Distinct structural features such as galactan, arabinan, xylan, and galacturonan motifs were present in the FT IR spectra. The intra- and intermolecular interactions of the polysaccharides, and their effect on the aggregation behavior, were shown by AFM. The composition and structural features of these polysaccharides were reflected in their prebiotic potential. Lactobacilli and Bifidobacteria were not able to utilize them, whereas members of Bacteroidetes showed utilization capacity. The obtained data suggest a high economic potential for this Opuntia species, with potential uses such as animal feed in arid areas, precise prebiotic, and symbiotic formulations, or as the carbon skeleton source in a green refinery. Our methodology can be used to evaluate the saccharides as the phenotype of interest, helping to guide the breeding strategy.
英文关键词	SEC ATR FT IR HPAEC-PAD methanolysis growth curves heteropolysaccharides mucilage Opuntia spp cactus nopal
类型	Article
语种	英语
开放获取类型	Green Published, gold
收录类别	SCI-E
WOS记录号	WOS:000945005200001
WOS关键词	INDICA L MILL ; CELL-WALLS ; POLYSACCHARIDES ; SPECTROSCOPY ; DIVERSITY
WOS类目	Biochemistry & Molecular Biology ; Chemistry, Multidisciplinary
WOS研究方向	Biochemistry & Molecular Biology ; Chemistry
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/397011
推荐引用方式 GB/T 7714	Cruz-Rubio, Jose Manuel,Riva, Alessandra,Cybulska, Justyna,et al. Neutral and Pectic Heteropolysaccharides Isolated from Opuntia joconostle Mucilage: Composition, Molecular Dimensions and Prebiotic Potential[J],2023,24(4).
APA	Cruz-Rubio, Jose Manuel.,Riva, Alessandra.,Cybulska, Justyna.,Zdunek, Artur.,Berry, David.,...&Maghuly, Fatemeh.(2023).Neutral and Pectic Heteropolysaccharides Isolated from Opuntia joconostle Mucilage: Composition, Molecular Dimensions and Prebiotic Potential.INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,24(4).
MLA	Cruz-Rubio, Jose Manuel,et al."Neutral and Pectic Heteropolysaccharides Isolated from Opuntia joconostle Mucilage: Composition, Molecular Dimensions and Prebiotic Potential".INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 24.4(2023).