干旱区生态环境知识资源中心(Arid): Improvement of mineral nutrition and rooting efficiency of Cannabis sativa L. for in vitro large-scale propagation

Arid

DOI	10.1007/s11627-022-10320-6
	Improvement of mineral nutrition and rooting efficiency of Cannabis sativa L. for in vitro large-scale propagation
	Zarei, Adel; Davis, Benjamin; Feyissa, Biruk A.; Dinani, Elham Tavakouli; Simons, Brigitte
通讯作者	Zarei, A
来源期刊	IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-PLANT
ISSN	1054-5476
EISSN	1475-2689
出版年	2023
卷号	59 期号:1 页码:95-105
英文摘要	Cannabis micropropagation is an effective method for producing clean plants in the new and expanding cannabis industry. However, existing protocols are not efficient enough to mass propagate for industry demands and to preserve large numbers of elite cultivars. This research was designed to validate optimal mineral nutrition for cannabis micropropagation. Currently, Murashige and Skoog (MS) basal salts are used in commercially available cannabis medium. Three mineral nutrient factors were defined from MS salts: ammonium nitrate, potassium nitrate, and mesonutrients (CaCl2 2H(2)O, KH2PO4, MgSO4 & BULL;7H(2)O). A total of 20 treatments (MS1-20) were designed, and each factor was varied over a range of concentrations. Leaf canopy area, shoot fresh weight, and multiplication number were measured to quantify the effects of mineral nutrients on the in vitro growth of three cannabis cultivars. All cultivars responded positively to increased nitrogen and mesonutrients (MS6) relative to MS medium. Plantlets grown on MS6 exhibited a significant increase in canopy area and fresh weight in two out of three studied cultivars. Additionally, an increase in multiplication number was noted on MS6 with an average increase in shoots per explant of 52% across three cultivars. The addition of potassium silicate (4.86 mM) to MS6 media reduced hyperhydricity symptoms in three hyperhydric cultivars. An improved root induction was observed on inert media, rockwool, and Oasis foam plugs when compared to standard culture media (1/2 MS containing 2.4 mu M IBA and MS containing 2 mu M m-topolin) that are routinely used as cannabis rooting media. Plantlets grown in rockwool resulted in healthy shoots and a high root fresh weight after 4 to 6 wk with mineral nutrient solution while plantlets grown on standard culture media did not produce roots during this period. Together, this study presents an end-to-end efficient procedure for cannabis micropropagation and preservation to be used in industrial large-scale production.
英文关键词	Cannabis sativa Photoautotrophic Micropropagation Hyperhydricity Medium optimization Mineral nutrition
类型	Article
语种	英语
收录类别	SCI-E
WOS记录号	WOS:000894725300001
WOS关键词	PHOTOAUTOTROPHIC MICROPROPAGATION ; INVITRO-PROPAGATION ; WATER-CONTENT ; GROWTH ; HEMP ; PROTOCOL ; REGENERATION ; SYSTEM
WOS类目	Plant Sciences ; Cell Biology ; Developmental Biology
WOS研究方向	Plant Sciences ; Cell Biology ; Developmental Biology
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/396915
推荐引用方式 GB/T 7714	Zarei, Adel,Davis, Benjamin,Feyissa, Biruk A.,et al. Improvement of mineral nutrition and rooting efficiency of Cannabis sativa L. for in vitro large-scale propagation[J],2023,59(1):95-105.
APA	Zarei, Adel,Davis, Benjamin,Feyissa, Biruk A.,Dinani, Elham Tavakouli,&Simons, Brigitte.(2023).Improvement of mineral nutrition and rooting efficiency of Cannabis sativa L. for in vitro large-scale propagation.IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-PLANT,59(1),95-105.
MLA	Zarei, Adel,et al."Improvement of mineral nutrition and rooting efficiency of Cannabis sativa L. for in vitro large-scale propagation".IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-PLANT 59.1(2023):95-105.