干旱区生态环境知识资源中心(Arid): Proteomic Analysis of the Cold Stress Response of Ammopiptanthus mongolicus Reveals the Role of AmCHIA in Its Cold Tolerance

Arid

DOI	10.3390/horticulturae9101114
	Proteomic Analysis of the Cold Stress Response of Ammopiptanthus mongolicus Reveals the Role of AmCHIA in Its Cold Tolerance
	Li, Xuting; Liu, Qi; Wu, Rongqi; Bing, Jie; Zheng, Lamei; Sumbur, Batu; Zhou, Yijun; Gao, Fei
通讯作者	Zhou, YJ ; Gao, F
来源期刊	HORTICULTURAE
EISSN	2311-7524
出版年	2023
卷号	9 期号:10
英文摘要	Ammopiptanthus mongolicus, a traditional ethnic medicinal herb, is a rare broad-leaved evergreen shrub in the arid region of central Asia that can survive under extremely low temperatures during winter. In this study, we investigated the cold stress response of A. mongolicus leaves using physiological and proteomic approaches. Cold stress treatment increased the relative electrolyte leakage, proline, and soluble sugar levels and decreased the chlorophyll content in A. mongolicus leaves. Moreover, 93 differentially abundant proteins (DAPs) were identified using two-dimensional electrophoresis, of which 79 were further confirmed via tandem mass spectrometric analysis. The predicted functions of DAPs were mainly associated with photosynthesis in chloroplasts, reactive oxygen species scavenging, defense, and protein synthesis, folding, and degradation. A. mongolicus chitinase A (AmCHIA) is a cold-induced apoplast protein whose transcription is upregulated under cold, osmotic, high-salinity, and mechanical stresses. Recombinant AmCHIA expressed in Escherichia coli exhibits chitinase activity. Here, AmCHIA expression enhanced the cold tolerance of E. coli cells, suggesting that it may contribute to the cold adaptation of A. mongolicus after cold treatment. The present study not only provides important data for understanding the cold stress responses in plants but also serves as a basis for further exploration of the biological functions of cold-induced proteins in A. mongolicus using genetic approaches.
英文关键词	Ammopiptanthus mongolicus leaf cold stress proteomics chitinase
类型	Article
语种	英语
开放获取类型	gold
收录类别	SCI-E
WOS记录号	WOS:001094142100001
WOS关键词	DROUGHT TOLERANCE ; CHILLING STRESS ; PROTEIN ; ANTIFREEZE ; EXPRESSION ; CHITINASE ; ACCLIMATION ; PROVIDES ; RUBISCO ; ROOTS
WOS类目	Horticulture
WOS研究方向	Agriculture
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/396850
推荐引用方式 GB/T 7714	Li, Xuting,Liu, Qi,Wu, Rongqi,et al. Proteomic Analysis of the Cold Stress Response of Ammopiptanthus mongolicus Reveals the Role of AmCHIA in Its Cold Tolerance[J],2023,9(10).
APA	Li, Xuting.,Liu, Qi.,Wu, Rongqi.,Bing, Jie.,Zheng, Lamei.,...&Gao, Fei.(2023).Proteomic Analysis of the Cold Stress Response of Ammopiptanthus mongolicus Reveals the Role of AmCHIA in Its Cold Tolerance.HORTICULTURAE,9(10).
MLA	Li, Xuting,et al."Proteomic Analysis of the Cold Stress Response of Ammopiptanthus mongolicus Reveals the Role of AmCHIA in Its Cold Tolerance".HORTICULTURAE 9.10(2023).