干旱区生态环境知识资源中心(Arid): Physiological and genomic insights into abiotic stress of halophilic archaeon Natrinema altunense 4.1R isolated from a saline ecosystem of Tunisian desert

Arid

DOI	10.1007/s10709-023-00182-0
	Physiological and genomic insights into abiotic stress of halophilic archaeon Natrinema altunense 4.1R isolated from a saline ecosystem of Tunisian desert
	Najjari, Afef; Boussetta, Ayoub; Youssef, Noha; Linares-Pasten, Javier A.; Mahjoubi, Mouna; Belloum, Rahma; Sghaier, Haitham; Cherif, Ameur; Ouzari, Hadda Imene
通讯作者	Linares-Pastén, JA
来源期刊	GENETICA
ISSN	0016-6707
EISSN	1573-6857
出版年	2023
卷号	151 期号:2 页码:133-152
英文摘要	Halophilic archaea are polyextremophiles with the ability to withstand fluctuations in salinity, high levels of ultraviolet radiation, and oxidative stress, allowing them to survive in a wide range of environments and making them an excellent model for astrobiological research. Natrinema altunense 4.1R is a halophilic archaeon isolated from the endorheic saline lake systems, Sebkhas, located in arid and semi-arid regions of Tunisia. It is an ecosystem characterized by periodic flooding from subsurface groundwater and fluctuating salinities. Here, we assess the physiological responses and genomic characterization of N. altunense 4.1R to UV-C radiation, as well as osmotic and oxidative stresses. Results showed that the 4.1R strain is able to survive up to 36% of salinity, up to 180 J/m(2) to UV-C radiation, and at 50 mM of H2O2, a resistance profile similar to Halobacterium salinarum, a strain often used as UV-C resistant model. In order to understand the genetic determinants of N. altunense 4.1R survival strategy, we sequenced and analyzed its genome. Results showed multiple gene copies of osmotic stress, oxidative stress, and DNA repair response mechanisms supporting its survivability at extreme salinities and radiations. Indeed, the 3D molecular structures of seven proteins related to responses to UV-C radiation (excinucleases UvrA, UvrB, and UvrC, and photolyase), saline stress (trehalose-6-phosphate synthase OtsA and trehalose-phosphatase OtsB), and oxidative stress (superoxide dismutase SOD) were constructed by homology modeling. This study extends the abiotic stress range for the species N. altunense and adds to the repertoire of UV and oxidative stress resistance genes generally known from haloarchaeon.
英文关键词	Haloarchaea Natrinema altunense UV-C radiation Oxidative stress Osmotic stress Genomic analysis Molecular modeling
类型	Article
语种	英语
开放获取类型	hybrid, Green Published
收录类别	SCI-E
WOS记录号	WOS:000939101500001
WOS关键词	MECHANOSENSITIVE CHANNELS ; OXIDATIVE STRESS ; RECA PROTEIN ; SALT-LAKE ; ANNOTATION ; BIOSYNTHESIS ; DIVERSITY ; GLUTATHIONE ; MECHANISMS ; STRATEGIES
WOS类目	Genetics & Heredity
WOS研究方向	Genetics & Heredity
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/396669
推荐引用方式 GB/T 7714	Najjari, Afef,Boussetta, Ayoub,Youssef, Noha,et al. Physiological and genomic insights into abiotic stress of halophilic archaeon Natrinema altunense 4.1R isolated from a saline ecosystem of Tunisian desert[J],2023,151(2):133-152.
APA	Najjari, Afef.,Boussetta, Ayoub.,Youssef, Noha.,Linares-Pasten, Javier A..,Mahjoubi, Mouna.,...&Ouzari, Hadda Imene.(2023).Physiological and genomic insights into abiotic stress of halophilic archaeon Natrinema altunense 4.1R isolated from a saline ecosystem of Tunisian desert.GENETICA,151(2),133-152.
MLA	Najjari, Afef,et al."Physiological and genomic insights into abiotic stress of halophilic archaeon Natrinema altunense 4.1R isolated from a saline ecosystem of Tunisian desert".GENETICA 151.2(2023):133-152.