干旱区生态环境知识资源中心(Arid): Genome-wide association study presents insights into the genetic architecture of drought tolerance in maize seedlings under field water-deficit conditions

Arid

DOI	10.3389/fpls.2023.1165582
	Genome-wide association study presents insights into the genetic architecture of drought tolerance in maize seedlings under field water-deficit conditions
	Chen, Shan; Dang, Dongdong; Liu, Yubo; Ji, Shuwen; Zheng, Hongjian; Zhao, Chenghao; Dong, Xiaomei; Li, Cong; Guan, Yuan; Zhang, Ao; Ruan, Yanye
通讯作者	Zhang, A ; Ruan, YY
来源期刊	FRONTIERS IN PLANT SCIENCE
ISSN	1664-462X
出版年	2023
卷号	14
英文摘要	Introduction: Drought stress is one of the most serious abiotic stresses leading to crop yield reduction. Due to the wide range of planting areas, the production of maize is particularly affected by global drought stress. The cultivation of drought-resistant maize varieties can achieve relatively high, stable yield in arid and semi-arid zones and in the erratic rainfall or occasional drought areas. Therefore, to a great degree, the adverse impact of drought on maize yield can be mitigated by developing drought-resistant or -tolerant varieties. However, the efficacy of traditional breeding solely relying on phenotypic selection is not adequate for the need of maize drought-resistant varieties. Revealing the genetic basis enables to guide the genetic improvement of maize drought tolerance. Methods: We utilized a maize association panel of 379 inbred lines with tropical, subtropical and temperate backgrounds to analyze the genetic structure of maize drought tolerance at seedling stage. We obtained the high quality 7837 SNPs from DArT's and 91,003 SNPs from GBS, and a resultant combination of 97,862 SNPs of GBS with DArT's. The maize population presented the lower heritabilities of the seedling emergence rate (ER), seedling plant height (SPH) and grain yield (GY) under field drought conditions. Results: GWAS analysis by MLM and BLINK models with the phenotypic data and 97862 SNPs revealed 15 variants that were significantly independent related to drought-resistant traits at the seedling stage above the threshold of P < 1.02 x 10-5. We found 15 candidate genes for drought resistance at the seedling stage that may involve in (1) metabolism (Zm00001d012176, Zm00001d012101, Zm00001d009488); ( 2) programmed cell death (Zm00001d053952); (3) transcriptional regulation (Zm00001d037771, Zm00001d053859, Zm00001d031861, Zm00001d038930, Zm00001d049400, Zm00001d045128 and Zm00001d043036); (4) autophagy (Zm00001d028417); and (5) cell growth and development (Zm00001d017495). The most of them in B73 maize line were shown to change the expression pattern in response to drought stress. These results provide useful information for understanding the genetic basis of drought stress tolerance of maize at seedling stage.
英文关键词	maize (Zea mays L.) genome-wide association study seedling stage field drought tolerance SNPs
类型	Article
语种	英语
开放获取类型	gold, Green Published
收录类别	SCI-E
WOS记录号	WOS:000990287800001
WOS关键词	TRANSCRIPTION FACTORS ; FUNCTIONAL-ANALYSIS ; BAX INHIBITOR-1 ; STRESS-RESPONSE ; EXPRESSION ; IDENTIFICATION ; RESISTANCE ; EXPANSIN ; PROTEIN ; RGLG1
WOS类目	Plant Sciences
WOS研究方向	Plant Sciences
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/396621
推荐引用方式 GB/T 7714	Chen, Shan,Dang, Dongdong,Liu, Yubo,et al. Genome-wide association study presents insights into the genetic architecture of drought tolerance in maize seedlings under field water-deficit conditions[J],2023,14.
APA	Chen, Shan.,Dang, Dongdong.,Liu, Yubo.,Ji, Shuwen.,Zheng, Hongjian.,...&Ruan, Yanye.(2023).Genome-wide association study presents insights into the genetic architecture of drought tolerance in maize seedlings under field water-deficit conditions.FRONTIERS IN PLANT SCIENCE,14.
MLA	Chen, Shan,et al."Genome-wide association study presents insights into the genetic architecture of drought tolerance in maize seedlings under field water-deficit conditions".FRONTIERS IN PLANT SCIENCE 14(2023).