干旱区生态环境知识资源中心(Arid): Impact of Green Revolution gene Rht-B1b on coleoptile length of wheat

Arid

DOI	10.3389/fpls.2023.1147019
	Impact of Green Revolution gene Rht-B1b on coleoptile length of wheat
	Xu, Dengan; Hao, Qianlin; Yang, Tingzhi; Lv, Xinru; Qin, Huimin; Wang, Yalin; Jia, Chenfei; Liu, Wenxing; Dai, Xuehuan; Zeng, Jianbin; Zhang, Hongsheng; He, Zhonghu; Xia, Xianchun; Cao, Shuanghe; Ma, Wujun
通讯作者	Ma, WJ
来源期刊	FRONTIERS IN PLANT SCIENCE
ISSN	1664-462X
出版年	2023
卷号	14
英文摘要	Wheat coleoptile is a sheath-like structure that helps to deliver the first leaf from embryo to the soil surface. Here, a RIL population consisting of 245 lines derived from Zhou 8425B x Chinese Spring cross was genotyped by the high-density Illumina iSelect 90K assay for coleoptile length (CL) QTL mapping. Three QTL for CL were mapped on chromosomes 2BL, 4BS and 4DS. Of them, two major QTL QCL.qau-4BS and QCL.qau-4DS were detected, which could explain 9.1%-22.2% of the phenotypic variances across environments on Rht-B1 and Rht-D1 loci, respectively. Several studies have reported that Rht-B1b may reduce the length of wheat CL but no study has been carried out at molecular level. In order to verify that the Rht-B1 gene is the functional gene for the 4B QTL, an overexpression line Rht-B1b-OE and a CRISPR/SpCas9 line Rht-B1b-KO were studied. The results showed that Rht-B1b overexpression could reduce the CL, while loss-of-function of Rht-B1b would increase the CL relative to that of the null transgenic plants (TNL). To dissect the underlying regulatory mechanism of Rht-B1b on CL, comparative RNA-Seq was conducted between Rht-B1b-OE and TNL. Transcriptome profiles revealed a few key pathways involving the function of Rht-B1b in coleoptile development, including phytohormones, circadian rhythm and starch and sucrose metabolism. Our findings may facilitate wheat breeding for longer coleoptiles to improve seedling early vigor for better penetration through the soil crust in arid regions.
英文关键词	wheat coleoptile length Rht-B1b QTL transcriptome
类型	Article
语种	英语
开放获取类型	gold, Green Published
收录类别	SCI-E
WOS记录号	WOS:000950139200001
WOS关键词	QUANTITATIVE TRAIT LOCI ; HEIGHT-REDUCING GENES ; DROUGHT-TOLERANCE ; SEEDLING GROWTH ; GENOMIC REGIONS ; PLANT HEIGHT ; NUMBER ; LIGHT ; ESTABLISHMENT ; GIBBERELLINS
WOS类目	Plant Sciences
WOS研究方向	Plant Sciences
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/396595
推荐引用方式 GB/T 7714	Xu, Dengan,Hao, Qianlin,Yang, Tingzhi,et al. Impact of Green Revolution gene Rht-B1b on coleoptile length of wheat[J],2023,14.
APA	Xu, Dengan.,Hao, Qianlin.,Yang, Tingzhi.,Lv, Xinru.,Qin, Huimin.,...&Ma, Wujun.(2023).Impact of Green Revolution gene Rht-B1b on coleoptile length of wheat.FRONTIERS IN PLANT SCIENCE,14.
MLA	Xu, Dengan,et al."Impact of Green Revolution gene Rht-B1b on coleoptile length of wheat".FRONTIERS IN PLANT SCIENCE 14(2023).