干旱区生态环境知识资源中心(Arid): Comparative transcriptome responses of leaf and root tissues to salt stress in wheat strains with different salinity tolerances

Arid

DOI	10.3389/fgene.2023.1015599
	Comparative transcriptome responses of leaf and root tissues to salt stress in wheat strains with different salinity tolerances
	Li, Jianfeng; Gao, Xin; Chen, Xunji; Fan, Zheru; Zhang, Yueqiang; Wang, Zhong; Shi, Jia; Wang, Chunsheng; Zhang, Hongzhi; Wang, Lihong; Zhao, Qi
通讯作者	Chen, XJ
来源期刊	FRONTIERS IN GENETICS
EISSN	1664-8021
出版年	2023
卷号	14
英文摘要	Background: Salinity stress is a major adverse environmental factor that can limit crop yield and restrict normal land use. The selection of salt-tolerant strains and elucidation of the underlying mechanisms by plant breeding scientists are urgently needed to increase agricultural production in arid and semi-arid regions.Results: In this study, we selected the salt-tolerant wheat (Triticum aestivum) strain ST9644 as a model to study differences in expression patterns between salt-tolerant and salt-sensitive strains. High-throughput RNA sequencing resulted in more than 359.10 Gb of clean data from 54 samples, with an average of 6.65 Gb per sample. Compared to the IWGSC reference annotation, we identified 50,096 new genes, 32,923 of which have functional annotations. Comparisons of abundances between salt-tolerant and salt-sensitive strains revealed 3,755, 5,504, and 4,344 genes that were differentially expressed at 0, 6, and 24 h, respectively, in root tissue under salt stress. KEGG pathway analysis of these genes showed that they were enriched for phenylpropanoid biosynthesis (ko00940), cysteine and methionine metabolism (ko00270), and glutathione metabolism (ko00480). We also applied weighted gene co-expression network analysis (WGCNA) analysis to determine the time course of root tissue response to salt stress and found that the acute response lasts > 6 h and ends before 12 h. We also identified key alternative splicing factors showing different splicing patterns in salt-sensitive and salt-tolerant strains; however, only few of them were differentially expressed in the two groups.Conclusion: Our results offer a better understanding of wheat salt tolerance and improve wheat breeding.
英文关键词	salt-tolerant wheat transcriptome WGCNA alternative splicing DEGs
类型	Article
语种	英语
开放获取类型	gold, Green Published
收录类别	SCI-E
WOS记录号	WOS:000946224600001
WOS关键词	ARABIDOPSIS ; PROTEINS ; MECHANISMS ; TRANSPORT ; HORMONES ; COMPLEX ; HISAT ; RICE
WOS类目	Genetics & Heredity
WOS研究方向	Genetics & Heredity
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/396542
推荐引用方式 GB/T 7714	Li, Jianfeng,Gao, Xin,Chen, Xunji,et al. Comparative transcriptome responses of leaf and root tissues to salt stress in wheat strains with different salinity tolerances[J],2023,14.
APA	Li, Jianfeng.,Gao, Xin.,Chen, Xunji.,Fan, Zheru.,Zhang, Yueqiang.,...&Zhao, Qi.(2023).Comparative transcriptome responses of leaf and root tissues to salt stress in wheat strains with different salinity tolerances.FRONTIERS IN GENETICS,14.
MLA	Li, Jianfeng,et al."Comparative transcriptome responses of leaf and root tissues to salt stress in wheat strains with different salinity tolerances".FRONTIERS IN GENETICS 14(2023).