干旱区生态环境知识资源中心(Arid): Transcriptome Analysis and Novel EST-SSR Marker Development for Pinus tabuliformis Seedlings from Four Provenances

Arid

DOI	10.3390/f14091810
	Transcriptome Analysis and Novel EST-SSR Marker Development for Pinus tabuliformis Seedlings from Four Provenances
	Wang, Ju; Guo, Shuai; Zhang, Yongxin; Zhang, Feng; Yun, Yufei; Zhang, Guosheng
通讯作者	Zhang, GS
来源期刊	FORESTS
EISSN	1999-4907
出版年	2023
卷号	14 期号:9
英文摘要	Chinese pine (Pinus tabuliformis) is a conifer species endemic to the temperate, warm temperate, and semi-arid regions of China. It has important ecological and economic values, but lacks suitable molecular markers for genetic studies. In this study, we collected open-pollinated progeny seeds from four provenances: Ningcheng (Nc), Qinyuan (Qy), Weichang (Wc), and Pingquan (Pq). We sequenced the transcriptomes of open-pollinated progeny seedlings from four provenances and annotated 18,244 unigenes. We analyzed the expression of genes involved in the auxin indole-3-acetic acid (IAA), cytokinin (CTK), and gibberellin (GA) signaling pathways among the provenances. Additionally, we detected 2811 expressed sequence tag simple sequence repeat (EST-SSR) loci in 2360 unigenes, with a frequency of 14.83% and an average of one locus per 14,556 base pairs (bp). We developed 10 polymorphic primers from 67 pairs and tested them on 56 samples from the four provenances. These primers exhibited moderate to high polymorphism and distinguished all samples clearly. Our study reveals variation in growth and development among open-pollinated progeny seedlings from different provenances of Chinese pine and provides novel markers for its genetic diversity study and marker-assisted breeding.
英文关键词	Chinese pine Pinus tabuliformis transcriptome simple repeat sequence
类型	Article
语种	英语
开放获取类型	Green Published, gold
收录类别	SCI-E
WOS记录号	WOS:001073637900001
WOS关键词	POPULATION ; SOFTWARE ; ALIGNMENT
WOS类目	Forestry
WOS研究方向	Forestry
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/396439
推荐引用方式 GB/T 7714	Wang, Ju,Guo, Shuai,Zhang, Yongxin,et al. Transcriptome Analysis and Novel EST-SSR Marker Development for Pinus tabuliformis Seedlings from Four Provenances[J],2023,14(9).
APA	Wang, Ju,Guo, Shuai,Zhang, Yongxin,Zhang, Feng,Yun, Yufei,&Zhang, Guosheng.(2023).Transcriptome Analysis and Novel EST-SSR Marker Development for Pinus tabuliformis Seedlings from Four Provenances.FORESTS,14(9).
MLA	Wang, Ju,et al."Transcriptome Analysis and Novel EST-SSR Marker Development for Pinus tabuliformis Seedlings from Four Provenances".FORESTS 14.9(2023).