干旱区生态环境知识资源中心(Arid): Screening of a Novel Fibrinolytic Enzyme-Producing Streptomyces from a Hyper-Arid Area and Optimization of Its Fibrinolytic Enzyme Production

Arid

DOI	10.3390/fermentation9050410
	Screening of a Novel Fibrinolytic Enzyme-Producing Streptomyces from a Hyper-Arid Area and Optimization of Its Fibrinolytic Enzyme Production
	He, Zixuan; Sun, Yang; Chu, Min; Zhu, Jing; Zhang, Yu; Tang, Qiyong; Osman, Ghenijan; Jiang, Ling; Zhang, Zhidong
通讯作者	Zhang, ZD
来源期刊	FERMENTATION-BASEL
EISSN	2311-5637
出版年	2023
卷号	9 期号:5
英文摘要	Fibrinolytic enzymes are a kind of proteolytic enzymes that can hydrolyze fibrin and dissolve blood clots. They could be used as a therapeutic agent for treating thrombosis. It is important for the treatment of cardiovascular disease to find and develop new thrombolytic drugs. In order to explore new fibrinolytic enzymes, a strain named 214L-11 with protease and fibrinolytic enzyme activity, which was isolated from the Flaming Mountain of Xinjiang Province, was screened using the skimmed milk plate, the blood powder agarose plate and the fibrin plate methods. Phylogenetic analyses showed that strain 214L-11 shared the highest similarity with Streptomyces fumanus NBRC 13042T (98.88%), which indicated that it represented a potential novel species in the Streptomyces genus. The fibrinolytic enzyme produced by 214L-11 displayed thrombolytic and anticoagulant activities, and it could degrade a single specific protein in the thrombus, thereby destroying the thrombus structure. The fermentation medium optimized through response surface methodology was 15 g/L soluble starch, g/L KNO3 0.58, 0.43 g/L peptone, 0.01 g/L FeSO4 center dot 7H(2)O, 0.5 g/L MgSO4 center dot 7H(2)O, 0.2 g/L Mn2+, 0.5 g/L NaCl and 1 L distilled water, pH 8, and the maximum amount of fibrinolytic enzyme produced by strain 214L-11 in the optimal fermentation medium was 1255.3 FU/mL. Overall, the fibrinolytic enzyme-producing strain was screened from the Flaming Mountain of Xinjiang for the first time, which provided a basis for further research and the development of new efficient and safe hemolytic drugs.
英文关键词	fibrinolytic enzyme Streptomyces anticoagulant response surface methodology
类型	Article
语种	英语
开放获取类型	gold
收录类别	SCI-E
WOS记录号	WOS:000996648800001
WOS关键词	BIOCHEMICAL-CHARACTERIZATION ; ENHANCED PRODUCTION ; SP NOV. ; PURIFICATION ; PROTEASE ; THROMBOLYSIS ; DIVERSITY ; MUSHROOM
WOS类目	Biotechnology & Applied Microbiology ; Food Science & Technology
WOS研究方向	Biotechnology & Applied Microbiology ; Food Science & Technology
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/396350
推荐引用方式 GB/T 7714	He, Zixuan,Sun, Yang,Chu, Min,et al. Screening of a Novel Fibrinolytic Enzyme-Producing Streptomyces from a Hyper-Arid Area and Optimization of Its Fibrinolytic Enzyme Production[J],2023,9(5).
APA	He, Zixuan.,Sun, Yang.,Chu, Min.,Zhu, Jing.,Zhang, Yu.,...&Zhang, Zhidong.(2023).Screening of a Novel Fibrinolytic Enzyme-Producing Streptomyces from a Hyper-Arid Area and Optimization of Its Fibrinolytic Enzyme Production.FERMENTATION-BASEL,9(5).
MLA	He, Zixuan,et al."Screening of a Novel Fibrinolytic Enzyme-Producing Streptomyces from a Hyper-Arid Area and Optimization of Its Fibrinolytic Enzyme Production".FERMENTATION-BASEL 9.5(2023).