干旱区生态环境知识资源中心(Arid): Integrated transcriptomics and metabolomics reveals that regulating biosynthesis and metabolism of HCN and GABA plays a key role in drought resistance of wild soybean

Arid

DOI	10.1016/j.envexpbot.2023.105505
	Integrated transcriptomics and metabolomics reveals that regulating biosynthesis and metabolism of HCN and GABA plays a key role in drought resistance of wild soybean
	Zhang, Shengmei; Hu, Yongjun; Wang, Yuming; Li, Mingxia; Han, Defu; Gao, Shujuan; Hu, Yunan; Guo, Jixun; Zhang, Tao; Shi, Lianxuan
通讯作者	Shi, LX
来源期刊	ENVIRONMENTAL AND EXPERIMENTAL BOTANY
ISSN	0098-8472
EISSN	1873-7307
出版年	2023
卷号	215
英文摘要	Wild soybeans [Glycine soja (Siebold & Zucc.)] grown in arid areas develop adaptive traits and they are important wild plant resources. In order to reveal the drought-tolerance mechanism in wild soybean, we studied the changes of growth parameters, photosynthetic physiology, and ion physiology of two wild soybean ecotypes under drought stress, and integratedly analyzed the transcriptome and metabolome changes in their leaves. Drought-tolerant wild soybean had greater root/shoot ratio, water use efficiency, and K+ and Ca2+ contents than common wild soybean. In drought-tolerant wild soybean, the gene encoding (R)-mandelonitrile lyase in the pathway of hydrogen cyanide (HCN) biosynthesis and metabolism and the genes encoding serine O-acetyltransferase and aminocyclopropanecarboxylate oxidase in the pathway of 1-aminocyclopropane-1-carboxylate oxidation to produce HCN were down-regulated. In HCN biosynthesis and metabolic pathways of droughttolerant wild soybean, the content of cysteine as a raw material was significantly reduced, while the content of 3-cyanoalanine as a product was significantly increased. In addition, drought-tolerant wild soybeans upregulated genes for glutamate synthase and glutamate decarboxylase during synthesis of 4-aminobutyric acid (GABA), accumulating aspartic acid, asparagine, glutamic acid, and GABA; and down-regulated the gene for 4aminobutyrate-pyruvate transaminase in GABA metabolism. Integrated analysis showed that drought-resistant wild soybean weakened the production of HCN and enhanced the utilization of HCN to avoid HCN toxicity under drought stress. At the same time, it also accumulated GABA to maintain carbon/nitrogen balance and amino acid metabolism. This study provides insights into the genetic mechanism of adaptation to drought by wild soybean, which will be helpful for screening of excellent wild plant resources.
英文关键词	Drought Metabolomics Wild soybean Transcriptomics 4-Aminobutyric acid 3-Cyanoalanine
类型	Article
语种	英语
收录类别	SCI-E
WOS记录号	WOS:001082515300001
WOS关键词	ACID ; STRATEGIES ; NITROGEN ; ETHYLENE
WOS类目	Plant Sciences ; Environmental Sciences
WOS研究方向	Plant Sciences ; Environmental Sciences & Ecology
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/396107
推荐引用方式 GB/T 7714	Zhang, Shengmei,Hu, Yongjun,Wang, Yuming,et al. Integrated transcriptomics and metabolomics reveals that regulating biosynthesis and metabolism of HCN and GABA plays a key role in drought resistance of wild soybean[J],2023,215.
APA	Zhang, Shengmei.,Hu, Yongjun.,Wang, Yuming.,Li, Mingxia.,Han, Defu.,...&Shi, Lianxuan.(2023).Integrated transcriptomics and metabolomics reveals that regulating biosynthesis and metabolism of HCN and GABA plays a key role in drought resistance of wild soybean.ENVIRONMENTAL AND EXPERIMENTAL BOTANY,215.
MLA	Zhang, Shengmei,et al."Integrated transcriptomics and metabolomics reveals that regulating biosynthesis and metabolism of HCN and GABA plays a key role in drought resistance of wild soybean".ENVIRONMENTAL AND EXPERIMENTAL BOTANY 215(2023).