干旱区生态环境知识资源中心(Arid): Ectopic expression of SpABR1 positively regulates drought stress tolerance through the ABA-dependent pathway and by promoting ROS scavenging in Arabidopsis

Arid

DOI	10.1016/j.envexpbot.2023.105491
	Ectopic expression of SpABR1 positively regulates drought stress tolerance through the ABA-dependent pathway and by promoting ROS scavenging in Arabidopsis
	Zhao, Jinna; Yang, Yanfei; Jia, Xiaoli; Meng, Huijing; Diao, Kehao; Zheng, Guangshun; Li, Tao; Li, Jianbo
通讯作者	Li, T
来源期刊	ENVIRONMENTAL AND EXPERIMENTAL BOTANY
ISSN	0098-8472
EISSN	1873-7307
出版年	2023
卷号	215
英文摘要	Glucosyltransferases, Rab-like GTPase activators and Myotubularins (GRAM) domain-containing proteins are essential for plant growth and reactions to abiotic stresses. The desert shrub willow Salix psammophila C. is native to arid and semi-arid areas and has strong tolerance of abiotic stresses. We previously identified a GRAM domain-containing protein, SpABR1, as a hub gene in the response of S. psammophila to drought stress. In this study, SpABR1 was isolated and functionally characterized. Analysis of expression patterns revealed highest SpABR1 expression in the roots. Additionally, GUS staining revealed the upregulation of SpABR1 in response to drought stress and ABA treatment. Overexpression of SpABR1 resulted in enhanced tolerance to drought stress and heightened ABA sensitivity. Compared to wild-type (WT) plants, plants with overexpressed SpABR1 exhibited increased activities of antioxidant enzymes under drought stress. In SpABR1 overexpression plants, there was higher expression of stress-related genes and ABA biosynthesis-related genes compared to WT plants under drought stress conditions. Additionally, results from a yeast one-hybrid screening assay and luciferase analysis revealed that SpNAC2 is an up-regulated gene that directly interacts with the promoter region of SpABR1 to activate its expression. Our findings revealed a positive role of SpABR1 in drought stress and provided insight into its regulatory network.
英文关键词	SpABR1 Drought Transgene Salix psammophila
类型	Article
语种	英语
收录类别	SCI-E
WOS记录号	WOS:001074684300001
WOS关键词	GRAM-DOMAIN ; CONFERS DROUGHT ; TRANSCRIPTION FACTOR ; GENE ; SALT ; RESPONSES ; COLD ; IDENTIFICATION ; MODULATION ; PROTEIN
WOS类目	Plant Sciences ; Environmental Sciences
WOS研究方向	Plant Sciences ; Environmental Sciences & Ecology
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/396106
推荐引用方式 GB/T 7714	Zhao, Jinna,Yang, Yanfei,Jia, Xiaoli,et al. Ectopic expression of SpABR1 positively regulates drought stress tolerance through the ABA-dependent pathway and by promoting ROS scavenging in Arabidopsis[J],2023,215.
APA	Zhao, Jinna.,Yang, Yanfei.,Jia, Xiaoli.,Meng, Huijing.,Diao, Kehao.,...&Li, Jianbo.(2023).Ectopic expression of SpABR1 positively regulates drought stress tolerance through the ABA-dependent pathway and by promoting ROS scavenging in Arabidopsis.ENVIRONMENTAL AND EXPERIMENTAL BOTANY,215.
MLA	Zhao, Jinna,et al."Ectopic expression of SpABR1 positively regulates drought stress tolerance through the ABA-dependent pathway and by promoting ROS scavenging in Arabidopsis".ENVIRONMENTAL AND EXPERIMENTAL BOTANY 215(2023).