干旱区生态环境知识资源中心(Arid): Detection of vertebrates from natural and artificial inland water bodies in a semi-arid habitat using eDNA from filtered, swept, and sediment samples

Arid

DOI	10.1002/ece3.10014
	Detection of vertebrates from natural and artificial inland water bodies in a semi-arid habitat using eDNA from filtered, swept, and sediment samples
	McDonald, Rupert; Bateman, Philip W.; Cooper, Christine; van der Heyde, Mieke; Mousavi-Derazmahalleh, Mahsa; Hedges, Brock A.; Guzik, Michelle T.; Nevill, Paul
通讯作者	Nevill, P
来源期刊	ECOLOGY AND EVOLUTION
ISSN	2045-7758
出版年	2023
卷号	13 期号:4
英文摘要	Biomonitoring is vital for establishing baseline data that is needed to identify and quantify ecological change and to inform management and conservation activities. However, biomonitoring and biodiversity assessment in arid environments, which are predicted to cover 56% of the Earth's land surface by 2100, can be prohibitively time consuming, expensive, and logistically challenging due to their often remote and inhospitable nature. Sampling of environmental DNA (eDNA) coupled with high-throughput sequencing is an emerging biodiversity assessment method. Here we explore the application of eDNA metabarcoding and various sampling approaches to estimate vertebrate richness and assemblage at human-constructed and natural water sources in a semi-arid region of Western Australia. Three sampling methods: sediment samples, filtering through a membrane with a pump, and membrane sweeping in the water body, were compared using two eDNA metabarcoding assays, 12S-V5 and 16smam, for 120 eDNA samples collected from four gnammas (gnamma: Australian Indigenous Noongar language term-granite rock pools) and four cattle troughs in the Great Western Woodlands, Western Australia. We detected higher vertebrate richness in samples from cattle troughs and found differences between assemblages detected in gnammas (more birds and amphibians) and cattle troughs (more mammals, including feral taxa). Total vertebrate richness was not different between swept and filtered samples, but all sampling methods yielded different assemblages. Our findings indicate that eDNA surveys in arid lands will benefit from collecting multiple samples at multiple water sources to avoid underestimating vertebrate richness. The high concentration of eDNA in small, isolated water bodies permits the use of sweep sampling that simplifies sample collection, processing, and storage, particularly when assessing vertebrate biodiversity across large spatial scales.
英文关键词	arid lands biodiversity biomonitoring environmental DNA Fauna surveys metabarcoding vertebrates
类型	Article
语种	英语
开放获取类型	gold, Green Published
收录类别	SCI-E
WOS记录号	WOS:000976773300001
WOS关键词	ENVIRONMENTAL DNA ; BIODIVERSITY ASSESSMENT ; ARID ENVIRONMENTS ; SPECIES RICHNESS ; IDENTIFICATION ; PERSISTENCE ; BACTEROIDES ; SELECTION ; POINTS
WOS类目	Ecology ; Evolutionary Biology
WOS研究方向	Environmental Sciences & Ecology ; Evolutionary Biology
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/396014
推荐引用方式 GB/T 7714	McDonald, Rupert,Bateman, Philip W.,Cooper, Christine,et al. Detection of vertebrates from natural and artificial inland water bodies in a semi-arid habitat using eDNA from filtered, swept, and sediment samples[J],2023,13(4).
APA	McDonald, Rupert.,Bateman, Philip W..,Cooper, Christine.,van der Heyde, Mieke.,Mousavi-Derazmahalleh, Mahsa.,...&Nevill, Paul.(2023).Detection of vertebrates from natural and artificial inland water bodies in a semi-arid habitat using eDNA from filtered, swept, and sediment samples.ECOLOGY AND EVOLUTION,13(4).
MLA	McDonald, Rupert,et al."Detection of vertebrates from natural and artificial inland water bodies in a semi-arid habitat using eDNA from filtered, swept, and sediment samples".ECOLOGY AND EVOLUTION 13.4(2023).