干旱区生态环境知识资源中心(Arid): Transcriptome Analysis of Embryogenic and Non-Embryogenic Callus of Picea Mongolica

Arid

DOI	10.3390/cimb45070332
	Transcriptome Analysis of Embryogenic and Non-Embryogenic Callus of Picea Mongolica
	Wang, Yaping; Wang, Hao; Bao, Wenquan; Sui, Mingming; Bai, Yu'e
通讯作者	Bai, YE
来源期刊	CURRENT ISSUES IN MOLECULAR BIOLOGY
ISSN	1467-3037
EISSN	1467-3045
出版年	2023
卷号	45 期号:7 页码:5232-5247
英文摘要	Picea mongolica is a rare tree species in China, which is of great significance in combating desertification and improving the harsh ecological environment. Due to the low rate of natural regeneration, high mortality, and susceptibility to pests and cold springs, Picea mongolica has gradually become extinct. At present, somatic embryogenesis (SE) is the most effective method of micro-proliferation in conifers, but the induction rate of embryogenic callus (EC) is low, and EC is difficult to differentiate from non-embryonic callus (NEC). Therefore, the EC and NEC of Picea mongolica were compared from the morphology, histological, physiological, and transcriptional levels, respectively. Morphological observation showed that the EC was white and transparent filamentous, while the NEC was compact and brownish-brown lumpy. Histological analyses showed that the NEC cells were large and loosely arranged; the nuclei attached to the edge of the cells were small; the cytoplasm was low; and the cell gap was large and irregular. In the EC, small cells, closely arranged cells, and a large nucleus and nucleolus were observed. Physiological studies showed significant differences in ROS-scavenging enzymes between the EC and NEC. Transcriptome profiling revealed that 13,267 differentially expressed genes (DEGs) were identified, 3682 were up-regulated, and 9585 were down-regulated. In total, 63 GO terms had significant enrichment, 32 DEGs in plant hormone signal transduction pathway were identified, and 502 different transcription factors (TFs) were characterized into 38 TF families. Meanwhile, we identified significant gene expression trends associated with somatic embryo development in plant hormones (AUX/IAA, YUCCA, LEA, etc.), stress (GST, HSP, GLP, etc.), phenylpropanoid metabolism (4CL, HCT, PAL, etc.), and transcription factors (AP2/ERF, MYB, WOX, etc.). In addition, nine genes were chosen for RT-qPCR, and the results were consistent with RNA-Seq data. This study revealed the changes in morphology, histology, physiology, and gene expression in the differentiation of NEC into EC and laid the foundation for finding the key genes to promote EC formation.
英文关键词	somatic embryogenesis callus transcriptomics morphology histocytology physiology Picea mongolica
类型	Article
语种	英语
开放获取类型	gold, Green Published
收录类别	SCI-E
WOS记录号	WOS:001035201300001
WOS关键词	IMPROVED SOMATIC EMBRYOGENESIS ; TO-EMBRYONIC TRANSITION ; MATURE ZYGOTIC EMBRYOS ; PLANT-REGENERATION ; ABSCISIC-ACID ; MICROSPORE EMBRYOGENESIS ; DIFFERENTIAL EXPRESSION ; PROTEIN ; GENES ; CULTURES
WOS类目	Biochemistry & Molecular Biology
WOS研究方向	Biochemistry & Molecular Biology
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/395834
推荐引用方式 GB/T 7714	Wang, Yaping,Wang, Hao,Bao, Wenquan,et al. Transcriptome Analysis of Embryogenic and Non-Embryogenic Callus of Picea Mongolica[J],2023,45(7):5232-5247.
APA	Wang, Yaping,Wang, Hao,Bao, Wenquan,Sui, Mingming,&Bai, Yu'e.(2023).Transcriptome Analysis of Embryogenic and Non-Embryogenic Callus of Picea Mongolica.CURRENT ISSUES IN MOLECULAR BIOLOGY,45(7),5232-5247.
MLA	Wang, Yaping,et al."Transcriptome Analysis of Embryogenic and Non-Embryogenic Callus of Picea Mongolica".CURRENT ISSUES IN MOLECULAR BIOLOGY 45.7(2023):5232-5247.